Immunology: Overview and Laboratory Manual

A two-in-one text providing teaching lab students with an overview of immunology as well as a lab manual complete with current standard exercises.
Section I of this book provides an overview of the immune system and immunity, and includes review questions, problem sets, case studies, inquiry-based questions, and more to provide students with a strong foundation in the field. Section II consists of twenty-two lab exercises focused on key concepts in immunology, such as antibody production, cell separation, cell function, immunoassays, Th1/Th2 cytokine detection, cell and tissue culture methods, and cell and molecular biology techniques. Appendices include safety information, suggested links and readings, and standard discipline processes, protocols, and instructions.

Provides inquiry-based exercises and standard exercises, allowing students to learn experimental design and hone critical thinking in theory, concepts, and data collection/interpretation Includes standard lab questions that promote hypothesis-based investigations Accessible writing, didactic features, and a descriptive glossary reinforce fundamental concepts for students

Auteur

Tobili Sam-Yellowe is a research scientist and educator with over 30 years experience in malaria research, vaccine discovery, and research design and analysis. Dr. Sam-Yellowe is Professor of Biology at Cleveland State University.

Contenu
Part I: Overview of Immunity.- Ch 1: Immunity.- Ch 2: Components of immunity.- Ch 3: Hematopoiesis.- Ch 4: The Immune system: pathogen sensing and detection.- Ch 5: Lymphoid organs.- Ch 6: Innate and Adaptive immunity.- Ch 7: Leukocyte homing, migration and recirculation.- Ch 8: Apoptosis.- Ch 9: Antigens.- Ch 10: B cell development, activation and immunoglobulin structure.- Ch 11: Immunoglobulin and T cell receptor gene rearrangements.- Ch 12: Major histocompatibility complex proteins.- Ch 13: T cell development and T cell receptor structure.- Ch 14: Cytokines.- Ch 15: Lymphocyte signals.- Ch 16: Complement fixation.- Ch 17: Cell mediated immunity.- Ch 18: Hypersensitivities.- Ch 19: Parasite Immunity.- Ch 20: Vaccines.- Ch 21: Transplantation.- Ch 22: Immune regulation and autoimmunity.- Ch 23: Immunodeficiencies.- Ch 24: Cancer.- Ch 25: Practice questions.- Ch 26: Study guide for exam. Part II. Laboratory Manual.- 27. Immunology laboratory policies.- 28. Instructions for notebook entries.- 29. Instructions for writing lab reports.- 30. Lab report topics.- 31. Exercise 1: Using a compound microscope.- 32. Exercise 2: Pipetting and dilution technique, identification of mouse lymphoid organs.- 33. Exercise 3: Blood Cell Preparation, Leukocyte differentiation and ABO Blood Typing.- 34. Exercise 4: Single Suspension of Mouse Spleen cells, Cell Viability Assays, and Identification of specific cells using surface antigens.- 35. Exercise 5: Isolation of mouse peritoneal macrophages: In vitro phagocytosis of bacterial cells.- 36. Exercise 6: Clearance of bacteria from mouse blood by the RES.- 37. Exercise 7: Hemolytic Plaque assay (The Jeme Plaque Assay).- 38. EXERCISE 8: Rabbit and Mouse Immunizations- Preparation of Polyclonal antibodies and screening assays.- 39. EXERCISE 9: Determination of Antibody Titer: Screening Techniques.- 40. EXERCISE 10: Immunodiffusion and Immunoelectrophoresis.- 41. EXERCISE 11: Protein Affinity Purification of Rabbit IgG.- 42. EXERCISE 12: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE).- 43. EXERCISE 13: Immunoblotting (Western Blotting).- 44. Exercise 14: Protein Assay.- 45. Exercise 15: Enzyme-linked Immunosorbent Assay (ELISA).- 46. EXERCISE 16: Enzyme-Linked Immunospot Assay (ELISPOT).- 47. EXERCISE 17: Immunofluorescence assay (IFA).- 48. EXERCISE 18: Flow Cytometry.- 49.EXERCISE 19: Complement Fixation Assays.- 50. Tissue Cell Culture Techniques.- 51. EXERCISE 20: Mitogen Induced Response of Lymphocytes:Detection of Band T cell Lymphocytes Using Immunofluorescence.- 52. Exercise 21: Induction of IL-2 Secretion from Mouse Lymphoma Cells.- 53. EXERCISE 22: Adoptive Transfer of Lymphocytes: Band T cell Cooperation for Antibody Production.- 54. EXERCISE 23: Macrophage Migration Inhibition.- 55. EXERCISE 24: Monoclonal Antibody Preparation.- 56. EXERCISE 25: Immunization of mice with a recombinant protein.- 57. EXERCISE 26: Polarization of T helper cells into TH1 subset.- 58. Exercise 27: Immunoprecipitation.- 59. EXERCISE 28: Induction of apoptosis in T lymphocytes and detection of apoptotic markers.- 60. EXERCISE 29: Cloning and sequencing of cDNAs of mouse variable regions of H and L chains of monoclonal antibodies.- Appendix.- Sample Practice Questions.