Testing for Genetic Manipulation in Plants

The debate over genetic manipulation and its use in plant impro- vement and protection has led to an increased demand for develo- ping methods for detecting and characterizing genetic manipula- tion in plants and plant products such as seeds and foods. This book is unique in presenting all relevant methods together in one volume: those for using and determining markers retained in genetically manipulated products as well as methods for elimina- ting marker genes and procedures for characterizing chromosomal aberrations in genetically manipulated plants.

Molecular Methods of Plant Analysis Concept of the Series The powerful recombinant DNA technology and related developments have had an enormous impact on molecular biology. Any treatment of plant analy sis must make use of these new methods. Developments have been so fast and the methods so powerful that the editors of Modern Methods of Plant Analy sis have now decided to rename the series Molecular Methods of Plant Analy sis. This will not change the general aims of the series, but best describes the thrust and content of the series as we go forward into the new millennium. This does not mean that all chapters a priori deal only with the methods of molecular biology, but rather that these methods are to be found in many chapters together with the more traditional methods of analysis which have seen recent advances. The numbering of the volumes of the series therefore continues on from 20, which is the most recently published volume under the title Modern Methods of Plant Analysis. As indicated for previous volumes, the methods to be found in Molecular Methods of Plant Analysis are described critically, with hints as to their limi tations, references to original papers and authors being given, and the chap ters written so that there is little need to consult other texts to carry out the All authors have been chosen because of their methods of analysis described.

Unique in presenting methods for detecting and characterizing genetic manipulation in plants and plant products Includes supplementary material: sn.pub/extras

Contenu
1 Selectable and Screenable Markers for Rice Transformation.- 2 Use of Green Fluorescent Protein to Detect Transformed Shoots.- 3 Luciferase Gene Expressed in Plants, Not in Agrobacterium.- 4 Use of ?-Glucuronidase (GUS) To Show Dehydration and High-Salt Gene Expression.- 5 Methods for Detecting Genetic Manipulation in Grain Legumes.- 6 Elimination of Selectable Marker Genes from Transgenic Crops.- 7 GST-MAT Vector for the Efficient and Practical Removal of Marker Genes from Transgenic Plants.- 8 Safety Assessment of Insect Protected Crops: Testing the Feeding Value of Bt Corn and Cotton Varieties in Poultry, Swine and Cattle.- 9 Safety Assessment of Genetically Modified Rice and Potatoes with Soybean Glycinin.- 10 Chromosomal and Genetic Aberrations in Transgenic Soybean.- 11 Transgenic Barley (Hordeum vulgare L.) and Chromosomal Variation.