Techniques in Light Microscopy

Auteur
Steven E. Ruzin was Director of the CNR Biological Imaging Facility at the University of California, Berkeley from 1989 to 2022 and has been Curator of the Golub Collection of antique microscopes since 2004. He is now Emeritus Professor at the University of California, Berkley.

Texte du rabat

The book describes in a simple and readable format the way microscopes magnify images, and all of the techniques that are available today. It covers all the existing epifluorescence techniques including super-resolution and provides historical context on inventions pertaining to the development of the light microscope.

Contenu

• Part I

• 1: Light Microscope Optics

• 2: Lens Aberrations

• 3: The Compound Microscope

• Part II

• 4: Transmitted Light Techniques

• 5: Polarized Light Microscopy

• 6: Phase Contrast Microscopy

• 7: Differential Interference Contrast Microscopy

• Part III

• 8: Epifluorescence Microscopy

• 9: Selective/Single Plane Imaging Microscopy

• 10: Confocal Microscopy

• 11: Two-Photon Microscopy

• 12: Restoration Microscopy

• 13: Super-Resolution Microscopy

• 14: Resolution

• 15: A Brief History of the Light Microscope

• Appendix I: Digital imaging and sampling

• Appendix II: Synopsis of transmitted light microscopy techniques

• Appendix III: Synopsis of epifluorescence microscopy techniques