Pseudomonas aeruginosa

This book aims to provide methods, protocols, and discussion topics for those who wish to examine in depth the molecular mechanisms of adaptation and versality of bacteria and would like to envisage their evolution responses in the fast changing Antropocene.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Pseudomonas aeruginosa: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Contenu

CRISPR/Cas9-based genome editing of Pseudomonas aeruginosa.- Investigating Pseudomonas aeruginosa Gene Function During Pathogenesis using Mobile-CRISPRi.- Engineering green-light-responsive heterologous gene expression in Pseudomonas.- Fluorescence-based evaluation of cyclic di-GMP levels in Pseudomonas aeruginosa.- Whole-cell biosensors for qualitative and quantitative analysis of quorum sensing signal molecules and the investigation of quorum quenching agents.- A Pseudomonas aeruginosa -suitable fluorescent reporter system for analyzing small RNA-mediated regulation of target mRNAs.- The Pseudomonas aeruginosa resistome: permanent and transient antibiotic resistance, an overview.- Biosensors for inducers of transient antibiotic resistance.- Pseudomonas aeruginosa soluble pyocins as antibacterial weapons.- Assays for studying Pseudomonas aeruginosa secreted proteases.- Single microcolony diffusion analysis in Pseudomonas aeruginosa biofilms.- Broad genome sequencing of environmental and clinical strains and genotyping.- Genome-scale analysis of the structure and function of RNA pathways and networks in Pseudomonas aeruginosa.- In-depth quantitative proteomics analysis of the Pseudomonas aeruginosa secretome. Improving the predictive value of preclinical mouse models of Pseudomonas aeruginosa respiratory infection to evaluate antibiotic efficacy.- Emerging in vitro models for the study of infection and pathogenesis of Pseudomonas aeruginosa and testing of antibacterial agents.