Protocols for Neural Cell Culture

Since the conception of this acclaimed series of volumes examining neural tissue culture, the expansion of neuroscience has continued to produce vital discoveries that utilize tissue culture methodologies. The expert contributors to the fourth edition of Protocols for Neural Cell Culture refine existing protocols and present the emerging new techniques and culture media formulations linked with the many advances in neuroscience. While highlighting updated experimental procedures for many of the classical neural tissue culture preps, the volume addresses topics such as regenerative medicine and the methods to grow and expand embryonic and adult neural stem cells. Essential techniques for the isolation, expansion, and cryopreservation of neural tissue from mouse, rat, and human sources are covered, as well as methods to assess aspects of cell function (calcium imaging) and cell death. With its detailed, step-by-step laboratory protocols, this volume provides the kind of detailed description and implementation advice that is crucial for getting optimal results.

Authoritative and cutting-edge, Protocols for Neural Cell Culture, Fourth Edition continues the previous editions' tradition of exceptional quality and thorough coverage in tissue culture protocols for today's neuroscience in order to inspire researchers in the field to further explore this imperative area of study.

Includes detailed protocols involving embryonic and adult stem cells, neuronal and glial preps, human and rodent cells, cellular assays, precursor cell expansion Serves as an easy-to-use reference for neuroscientists to establish tissue culture preps of neural tissue to answer specific research questions Provides the neuroscience community with procedures to isolate and culture the desired cell type from the fetal or adult nervous system Includes supplementary material: sn.pub/extras

Contenu
Neurosphere and Neural Colony-Forming Cell Assays.- Directed Neuronal Differentiation of Embryonic and Adult-Derived Neurosphere Cells.- Culture and Differentiation of Human Neural Stem Cells.- Neural Differentiation of Human Embryonic Stem Cells.- Isolation and Culture of Primary Human CNS Neural Cells.- Bioengineering Protocols for Neural Precursor Cell Expansion.- Intracellular Calcium Assays in Dissociated Primary Cortical Neurons.- Dissociated Hippocampal Cultures.- Primary Sensory and Motor Neuron Cultures.- Retinal Cell and Tissue Culture.- Preparation of Normal and Reactive Astrocyte Cultures.- Oligodendrocyte Progenitor Cell Culture.- Isolation of Microglia Subpopulations.- Microglia from Progenitor Cells in Mouse Neopallium.- Primary Schwann Cell Cultures.- Primary Dissociated Astrocyte and Neuron Co-culture.- Cerebellar Slice Cultures.- Hippocampal Slice Cultures.- Molecular Substrates for Growing Neurons in Culture.- Guidance and Outgrowth Assays for Embryonic Thalamic Axons.- Detection of Cell Death in Neuronal Cultures.- Live Imaging of Neural Cell Functions.- Tissue Culture Procedures and Tips.