Protocols for Micropropagation of Woody Trees and Fruits

Micropropagation has become a reliable and routine approach for large-scale rapid plant multiplication, which is based on plant cell, tissue and organ culture on well defined tissue culture media under aseptic conditions. A lot of research efforts are being made to develop and refine micropropagation methods and culture media for large-scale plant multiplication of several number of plant species. However, many forest and fruit tree species still remain recalcitrant to in vitro culture and require highly specific culture conditions for plant growth and development. The recent challenges on plant cell cycle regulation and the presented potential molecular mechanisms of recalcitrance are providing excellent background for understanding on totipotency and what is more development of micropropagation protocols. For large-scale in vitro plant production the important attributes are the quality, cost effectiveness, maintenance of genetic fidelity, and long-term storage. The need for appropriate in vitro plant regeneration methods for woody plants, including both forest and fruit trees, is still overwhelming in order to overcome problems facing micropropagation such as somaclonal variation, recalcitrant rooting, hyperhydricity, polyphenols, loss of material during hardening and quality of plant material. Moreover, micropropagation may be utilized, in basic research, in production of virus-free planting material, cryopreservation of endangered and elite woody species, applications in tree breeding and reforestation.

Presents step-wise protocols on micropropagation Provides information of organogenesis approach for plant multiplication Easy to follow step by step roptocols for numerous woody plants

Contenu
Section A.- Totipotency and the cell cycle.- Micropropagation via organogenesis in slash pine.- Micropropagation of Coast Redwood (Sequoia Sempervirens).- Micropropagation of Pinus pinea L.- Micropropagation of Pinus armandii var. Amamiana.- Organogenesis and Cryopreservation of Juvenile Radiata Pine.- Genetic Fidelity Analyses of In Vitro Propagated Cork Oak (Quercus suber L.).- Protocol for Micropropagation of Quercus spp.- Micropropagation of Mediterranean cypress (Cupressus sempervirens L.).- In vitro Shoot Development of Taxus Wallichiana Zucc., a Valuable Medicinal Plant.- Micropropagation of Yew (Taxus Baccata L.).- Micropropagation of Larix Species via Organogenesis.- Propagation of Selected Pinus Genotypes Regardless of Age.- Root Induction of Pinus Sylvestris L. Hypocotyl Cuttings using Specific Ectomycorrhizal Fungi in Vitro.- Micropropagation of Betula Pendula Roth including Genetically modified Material.- Protocol for Doubled-Haploid Micropropagation in Quercus suber L. and Assisted Verification.- In vitro Propagation of Fraxinus Species.- Micropropagation of Black Locust (Robinia Pseudoacacia L.).- Albizia Odoratissima L.F. (Benth) Micropropagation.- Micropropagation of Salix caprea L.- Micropropagation of Cedrela fissilis Vell. (Meliaceae).- Micropropagation of Mature Trees of Ulmus Glabra, Ulmus Minor and Ulmus Laevis.- Section B.- Micrografting in Grapevine (Vitis spp.).- Micrografting Grapevine for Virus Indexing.- Apricot micropropagation.- In vitro conservation and Micropropagation of Breadfruit (Artocarpus Altilis, Moracea).- Micrografting of Pistachio (Pistacia vera L. cv. Siirt).- Protocol for Micropropagation of Castanea Sativa.- Micropropagation of Cashew (Anacardium occidentale L.).- In vitro Mutagenesis and Mutant Multiplication.- In vitro propagation of Nutmeg, Myristica Fragrans Houtt.- Micropropagation of Elite Neem Tree (Azadirachta Indica A. Juss.).- Micropropagation Protocol for Microspore Embryogenesis in Olea Europaea L.- Micropropagation of Prunus Domestica and Prunus Salicina using Mature Seeds.- Micropropagation of Juglans regia L.- Tissue culture propagation of Mongolian cherry (Prunus fruticosa L.) and Nanking cherry (Prunus Tomentosa L.).- Micropropagation of Fig Tree (Ficus carica sp).- High frequency shoot formation of yellow passion fruit (Passiflora edulis F. flavicarpa) via thin Cell Layer (TCL) Technology.- Micropropagation of Calabash tree Crescentia Cujete L.- Micropropagation of Papaya (Carica Papaya L.).- Section C.- Protocol for Micropropagation of Selected Vaccinium spp.- Protocol for Micropropagation of Vaccinium Vitis-Idaea L.- Micropropagation of Bamboo Species Through Axillary Shoot Proliferation.- In vitro Culture Of Tree Peony Through Axillary Budding.- Micropropagation of pineapple, Ananas comosus (L.) Merr.- Date Palm Phoenix dactylifera L. Micropropagation.- Light-Emitting Diodes as an Effective Lighting Source for In Vitro Banana Culture.- In vitro Mutagenesis in Banana (Musa spp.) using Gamma Irradiation.