Protein Acetylation

This Methods in Molecular Biology(TM) book covers techniques for identifying protein acetylation, column- and gel electrophoresis-based approaches, computational prediction and biological response to protein acetylation. Includes protocols and troubleshooting tips.

Thousands of proteins have been identified to be acetylated. Immense research power has been dedicated to experiments to solve the biological implications of each and every protein acetylation. Two particular sites of protein acetylation have been described intensively: the N-terminal methionine residue of a nascent protein and lysine residues within a protein. In Protein Acetylation: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study protein acetylation. These include methods and techniques for identification of protein acetylation, column- and gel electrophoresis-based approaches, computationally prediction, and the biological response to protein acetylation. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Protein Acetylation: Methods and Protocols seeks to aid scientists in the further study of the technical aspects involved in understanding protein acetylation.

Features cutting-edge laboratory protocols dealing with protein acetylation Addresses the functional consequences of protein acetylation Contains expert tips on troubleshooting and avoiding known pitfalls Includes supplementary material: sn.pub/extras

Contenu

Validation of Protein Acetylation by Mass Spectrometry.- Application of the CIRAD Mass Spectrometry Approach for Lysine Acetylation Site Discovery.- Application of the MIDAS Approach for Analysis of Lysine Acetylation Sites.- Application of High Content Biology to Yield Quantitative Spatial Proteomic Information on Protein Acetylations.- Towards the N-terminal Acetylome: An N-terminal Acetylated Peptide Enrichment Method Using CNBr-Activated Sepharose Resin.- Identification and Analysis of O -acetylated Sialoglycoproteins.- HPLC-based Quantification of in vitro N-terminal Acetylation.- Separation and Purification of Multiply Acetylated Proteins using Cation-exchange Chromatography.- In-gel N-acetylation for the Quantification of the Degree of Protein in vivo N-terminal Acetylation.- Computational Prediction of Lysine Acetylation Proteome-wide.- Generation and Characterization of Pan-specific Anti-acetyllysine Antibody.- Using Functional Proteome Microarrays to Study Protein Lysine Acetylation.- Quantitation of Nucleosome Acetylation and other Histone Post-Translational Modifications Using Microscale NU-ELISA.- Preparing Semisynthetic and Fully Synthetic Histones H3 and H4 to Modify the Nucleosome Core.- Production of Amino-terminally Acetylated Recombinant Proteins in E. coli.- Identification of Lysine Acetyltransferase Substrates using Bioorthogonal Chemical Proteomics.- Non-radioactive in-vitro Assays for Histone Deacetylases.- The Fluorescence-Based Acetylation Assay Using Thiol-Sensitive Probes.- Analysis of Protein Acetyltransferase Structure-function Relation by Surface-Enhanced Raman Scattering (SERS): A Tool to Screen and Characterize Small Molecule Modulators.