Plant Cell Wall Analysis

Modern Methods of Plant Analysis When the handbook Modern Methods of Plant Analysis, was first introduced in 1954, the considerations were: 1. the dependence of scientific progress in biology on the improvement of existing and the introduction of new methods; 2. the difficulty in finding many new analytical methods in specialized journals which are normally not accessible to experimental plant biologists; 3. the fact that in the methods sections of papers the description of methods is frequently so compact, or even sometimes to incomplete, that it is difficult to reproduce experiments. These considerations still stand today. The series was highly successful, seven volumes appearing between 1956 and 1964. Since there is still today a demand for the old series, the publisher has decided to resume publication of Modern Methods of Plant Analysis. It is hoped that the New Series will be just as acceptable to those working in plant sciences and related fields as the early volumes undoubtedly were. It is difficult to single out the major reasons for the success of any publication, but we believe that the methods published in the first series were up-to-date at the time and presented in a way that made description, as applied to plant material, complete in itself with little need to consult other publications. Contribution authors have attempted to follow these guidelines in this New Series of volumes. Editorial The earlier series of Modern Methods of Plant Analysis was initiated by Michel V.

Texte du rabat

This book brings together techniques dealing with those parts of plant cells which have often been thought of as being extremely difficult to analyse. Cell wall porosity adhesives, proteins, chitin, lignin, oligosaccharides, pectins and other polymers are all the subject of analytical procedures in this volume. Wherever possible, the methods stand alone with little or no necessity to consult other works to carry out the methods put forward. Each chapter has been written by experts in the field using the most up-to-date analysis techniques.

Contenu
Fractionation of Cell Wall Components.- 1 Introduction.- 2 Polysaccharides.- 3 Proteins and Glycoproteins.- 4 Lignins.- References.- Isolation and Analysis of Cell Wall Polymers from Olive Pulp.- 1 Introduction.- 2 Isolation of Cell Walls from Olive Pulp.- 3 Sequential Extraction of Cell Wall Polymers.- 4 Fractionation of the Extracted Cell Wall Polysaccharides.- 5 Chemical and Spectroscopic Analysis.- 6 Concluding Remarks.- References.- Determination of Cell Wall Autolysis.- 1 Introduction.- 2 Factors Altering Cell Wall Isolation and Autolysis.- 3 Isolation of Active Cell Walls.- 4 Isolation of Inactive Cell Walls (Controls).- 5 Autolysis Incubations.- 6 Analysis of Autolysis Products.- 7 Conclusions.- References.- Cell Wall Porosity and Its Determination.- 1 Introduction.- 2 Microscopic Visualization of Wall Pores.- 3 Bulk Exclusion Techniques.- 4 Tracer Techniques: Uptake of Molecules or Particles.- 5 Conclusions.- References.- Analysis of Chitin Biosynthesis.- 1 Introduction.- 2 Structure of Chitin.- 3 The Enzymatic Synthesis of Chitin.- 4 Preparation of Chitin Synthase.- 5 Product Characterization of Chitin Synthase.- 6 Polymerization and Crystallization.- 7 Fungal Chitin Synthase Genes.- 8 Morphogenetic Roles of Chitin Synthases.- 9 Conclusions.- References.- Analysis of Plant-Substratum Adhesives.- 1 Introduction.- 2 Case Studies of Cell-Substratum Adhesion.- 3 Methods for the Analysis of Cell-Substratum Adhesives.- 4 Conclusions.- References.- Biochemical, Immunological and Molecular Analyses of Extensin.- 1 Introduction.- 2 Biochemical Characterization of Extensin.- 3 Immunological Detection of Extensin.- 4 Molecular Characterization of Extensin.- 5 Summary.- References.- Analysis of Pectin Structure by HPAEC-PAD.- 1 Introduction.- 2 Pectin HPLC Separations.- 3Pectin Analysis in Ripening Peach Fruit.- 4 Conclusions and Future Directions.- References.- Characterization of Oligosaccharides Derived from Plant Cell Wall Polysaccharides by On-Line High-Performance Anion-Exchange Chromatography Thermospray Mass Spectrometry.- 1 Introduction.- 2 Methods.- 3 HPAEC in Oligosaccharide Analysis.- 4 General Experimental Considerations Related to HPAEC-MS.- 5 Application of HPAEC-MS in Oligosaccharide Characterization.- 6 Conclusions and Perspectives.- References.- Analysis of Pectin Methyl Esterases.- 1 Introduction.- 2 Estimation and Detection of PME Activities.- 3 PME Localization.- 4 PME Extraction and Purification.- 5 PME Properties.- 6 Roles of PMEs.- 7 Conclusions and Perspectives.- References.- Probing the Subunit Composition and Topology of Plasma Membrane-Bound (1,3)-?-Glucan (Callose) Synthases.- 1 Introduction.- 2 Membrane Isolation, Enzyme Assay, and Solubilization.- 3 Callose Synthase Topology, Purification, and Subunit Composition.- 4 Biochemical Characterization of Integral Plant Plasma Membrane Proteins.- 5 Summary.- References.