High-Throughput Screening Methods in Toxicity Testing

Informationen zum Autor PABLO STEINBERG, PhD , is Full Professor for Food Toxicology and Replacement/Complementary Methods to Animal Testing as well as Director of the Institute for Food Toxicology and Analytical Chemistry at the University of Veterinary Medicine Hannover. Prior to that, Dr. Steinberg was director of the Institute of Nutritional Science at the University of Potsdam. He has also held research positions at the University of Mainz. Klappentext Explores the benefits and limitations of the latest high-throughput screening methodsWith its expert coverage of high-throughput in vitro screening methods for toxicity testing, this book makes it possible for researchers to accelerate and streamline the evaluation and risk assessment of chemicals and drugs for toxicity. Moreover, it enables them to comply with the latest standards set forth by the U.S. National Research Council's "Toxicity Testing in the 21st Century: A Vision and Strategy" and the E.U.'s REACH legislation. Readers will discover a variety of state-of-the-science, high-throughput screening methods presented by a group of leading authorities in toxicology and toxicity testing.High-Throughput Screening Methods in Toxicity Testing is divided into five parts: General aspects, including predicting the toxicity potential of chemicals and drugs via high-throughput bioactivity profiling Assessing different cytotoxicity endpoints Assessing DNA damage and carcinogenesis Assessing reproductive toxicity, cardiotoxicity, and haematotoxicity* Assessing drug metabolism and receptor-related toxicityEach chapter describes method principles and includes detailed information about data generation, data analysis, and applications in risk assessment. The authors not only enumerate the advantages of each high-throughput method over comparable conventional methods, but also point out the high-throughput method's limitations and potential pitfalls. In addition, the authors describe current research efforts to make high-throughput toxicity screening even more cost effective and streamlined.Throughout the book, readers will find plenty of figures and illustrations to help them understand and perform the latest high-throughput toxicity screening methods.This book is ideal for toxicologists and other researchers who need to implement high-throughput screening methods for toxicity testing in their laboratories as well as for researchers who need to evaluate the data generated by these methods. Zusammenfassung High-throughput assays are part of a paradigm shift, to more cost-effective and reliable methods, in the testing of chemical and drug safety. Inhaltsverzeichnis PREFACE ix CONTRIBUTORS xi PART I GENERAL ASPECTS 1 ToxCast: Predicting Toxicity Potential Through High-Throughput Bioactivity Profiling 3 Keith A. Houck, Ann M. Richard, Richard S. Judson, Matthew T. Martin, David M. Reif, and Imran Shah 2 High-Throughput Toxicity Testing in Drug Development: Aims, Strategies, and Novel Trends 33 Willem G.E.J. Schoonen, Walter M.A. Westerink, Femke M. van de Water, and Horbach G. Jean 3 Incorporating Human Dosimetry and Exposure Information with High-Throughput Screening Data in Chemical Toxicity Assessment 77 Barbara A. Wetmore and Russell S. Thomas 4 The Use of Human Embryonic Stem Cells in High-Throughput Toxicity Assays 97 Xin Huang, Dan-yan Zhu, and Yi-jia Lou PART II HIGH-THROUGHPUT ASSAYS TO ASSESS DIFFERENT CYTOTOXICITY ENDPOINTS 5 High-Throughput Screening Assays for the Assessment of Cytotoxicity 109 Andrew L. Niles, Richard A. Moravec, Tracy J. Worzella, Nathan J. Evans, and Terry L. Riss 6 High-Throughput Flow Cytometry Analysis of Apoptosis 129 Francesca de Giorgi and Franc ois Ichas 7 High Content Imaging-Based Screening for Cellular Toxicity Pathways...

Auteur

PABLO STEINBERG, PhD, is Full Professor for Food Toxicology and Replacement/Complementary Methods to Animal Testing as well as Director of the Institute for Food Toxicology and Analytical Chemistry at the University of Veterinary Medicine Hannover. Prior to that, Dr. Steinberg was director of the Institute of Nutritional Science at the University of Potsdam. He has also held research positions at the University of Mainz.

Texte du rabat
Explores the benefits and limitations of the latest high-throughput screening methods With its expert coverage of high-throughput in vitro screening methods for toxicity testing, this book makes it possible for researchers to accelerate and streamline the evaluation and risk assessment of chemicals and drugs for toxicity. Moreover, it enables them to comply with the latest standards set forth by the U.S. National Research Council's "Toxicity Testing in the 21st Century: A Vision and Strategy" and the E.U.'s REACH legislation. Readers will discover a variety of state-of-the-science, high-throughput screening methods presented by a group of leading authorities in toxicology and toxicity testing. High-Throughput Screening Methods in Toxicity Testing is divided into five parts: General aspects, including predicting the toxicity potential of chemicals and drugs via high-throughput bioactivity profiling Assessing different cytotoxicity endpoints Assessing DNA damage and carcinogenesis Assessing reproductive toxicity, cardiotoxicity, and haematotoxicity * Assessing drug metabolism and receptor-related toxicity Each chapter describes method principles and includes detailed information about data generation, data analysis, and applications in risk assessment. The authors not only enumerate the advantages of each high-throughput method over comparable conventional methods, but also point out the high-throughput method's limitations and potential pitfalls. In addition, the authors describe current research efforts to make high-throughput toxicity screening even more cost effective and streamlined. Throughout the book, readers will find plenty of figures and illustrations to help them understand and perform the latest high-throughput toxicity screening methods. This book is ideal for toxicologists and other researchers who need to implement high-throughput screening methods for toxicity testing in their laboratories as well as for researchers who need to evaluate the data generated by these methods.

Résumé
High-throughput assays are part of a paradigm shift, to more cost-effective and reliable methods, in the testing of chemical and drug safety.

Contenu

PREFACE ix

CONTRIBUTORS xi

PART I GENERAL ASPECTS

1 ToxCast: Predicting Toxicity Potential Through High-Throughput Bioactivity Profiling 3
Keith A. Houck, Ann M. Richard, Richard S. Judson, Matthew T. Martin, David M. Reif, and Imran Shah

2 High-Throughput Toxicity Testing in Drug Development: Aims, Strategies, and Novel Trends 33
Willem G.E.J. Schoonen, Walter M.A. Westerink, Femke M. van de Water, and Horbach G. Jean

3 Incorporating Human Dosimetry and Exposure Information with High-Throughput Screening Data in Chemical Toxicity Assessment 77
Barbara A. Wetmore and Russell S. Thomas

4 The Use of Human Embryonic Stem Cells in High-Throughput Toxicity Assays 97
Xin Huang, Dan-yan Zhu, and Yi-jia Lou

PART II HIGH-THROUGHPUT ASSAYS TO ASSESS DIFFERENT CYTOTOXICITY ENDPOINTS

5 High-Throughput Screening Assays for the Assessment of Cytotoxicity 109
Andrew L. Niles, Richard A. Moravec, Tracy J. Worzella, Nathan J. Evans, and Terry L. Riss

6 High-Throughput Flow Cytometry Analysis of Apoptosis 129
Francesca de Giorgi and Franc ois Ichas

7 High Content Imaging-Based Screening for Cellular Toxicity Pathways 143
Bram Herpers and Bob van de Water

8 The Keratinosens Assay: A High-Throughput Screening Assay to Assess Chemical Skin Sensitization 159
Andreas Natsch

9 High-Throughput Screening Assays to Assess Chemical Phototoxicity 177
Satomi Onoue, Yoshiki Seto, and Shizuo Yamada

**PART III HIGH-THROUGHPUT ASSAYS…