Molecular Biomethods Handbook

Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. This book provided theory, outlines of practical procedures, and applications for a range of techniques.

Comprehensive collection of wide-ranging molecular and biochemical techniques frequently used in medical and clinical research laboratories around the world Covers a wide array of techniques and protocols used for diagnostics Includes step-by-step reproducible laboratory protocols written by experts in the field and includes a notes section that outlines tricks of the trade Provides in-depth understanding of the theory, practice and application of each technique

Contenu
Nucleic Acid Methods.- The Manipulation of Nucleic Acids.- Restriction Enzymes.- Principles and Medical Applications of the Polymerase Chain Reaction.- Probe Design, Production, and Applications.- Southern Blotting as a Diagnostic Method.- Capillary Electrophoresis of DNA.- Denaturing High-Performance Liquid Chromatography (DHPLC) for Nucleic Acid Analysis.- Denaturing Gradient Gel Electrophoresis (DGGE).- Single Strand Conformation Polymorphism (SSCP) Analysis.- Randomly Amplified Polymorphic DNA (RAPD).- Quantification of mRNA Using Real Time RT-PCR.- Quantitative Analysis of DNA Sequences by PCR and Solid-Phase Minisequencing.- Multiplex Amplifiable Probe Hybridization (MAPH).- Gene Expression Profiling.- Comparative Genomic Hybridization in Clinical and Medical Research.- Subtractive Hybridization.- Fluorescence In Situ Hybridization.- Quantitative Trait Locus Mapping to Identify Genes for Complex Traits in Mice.- cDNA Microarrays.- Mapping Techniques.- Single Nucleotide Polymorphisms.- Gene/Protein Sequence Analysis.- Protein and Cell Methods.- Protein Electrophoresis.- Protein Blotting.- Capillary Electrophoresis of Proteins.- Autoradiography and Fluorography.- Mass Spectrometry of Proteins and Peptides.- Post-Translational Modif ications of Proteins.- Protein Microarray Technology.- ProteinProtein Interactions.- Glycoprotein Analysis.- Solid-Phase Peptide Synthesis.- Monoclonal Antibodies.- Antibody Phage Display.- Protein Engineering.- Directed Protein Evolution.- Enzyme Linked Immunosorbent Assay (ELISA).- Epitope Mapping.- Quantum Dots.- Ion-Exchange Chromatography.- Size-Exclusion Chromatography.- Hydrophobic Interaction Chromatography.- Affinity Chromatography.- High Performance Liquid Chromatography (HPLC) of Peptides and Proteins.- Amino Acid Analysis.- Surface Plasmon Resonance.- Macromolecular Crystallography.- Microchip Devices for Bioanalysis.- Mammalian Cell Culture.- Plant Tissue Culture.- Stem Cells and Regenerative Medicine.- Cryopreservation.- Magnetic Resonance Imaging.- Electron Microscopy.- Confocal Microscopy.- Laser Microdissection.- Biomedical Uses of Flow Cytometry.- Immunomicroscopy.- In Situ Hybridization.- High Throughput Screening.