Models for Assessing Drug Absorption and Metabolism

Pharmaceutical scientists in industry and academia will appreciate this single reference for its detailed experimental procedures for conducting biopharmaceutical studies. This well-illustrated guide allows them to establish, validate, and implement commonly used in situ and in vitro model systems. Chapters provide ready access to these methodologies for studies of the intestinal, buccal, nasal and respiratory, vaginal, ocular, and dermal epithelium as well as the endothelial and elimination barriers.

Contenu
1 General Principles in the Characterization and Use of Model Systems for Biopharmaceutical Studies.- 2 Methods for Evaluating Intestinal Permeability and Metabolism in Vitro.- 3 Cultured Intestinal Epithelial Cell Models.- 4 Intestinal Rings and Isolated Intestinal Mucosal Cells.- 5 Models of Drug Absorption in Situ and in Conscious Animals.- 6 Model Systems for Intestinal Lymphatic Transport Studies.- 7 Buccal Tissues and Cell Culture.- 8 Isolated Hepatocytes.- 9 Cultured Rat Hepatocytes.- 10 Isolated Perfused Liver.- 11 Isolated Renal Brush Border and Basolateral Membrane Vesicles and Cultured Renal Cells.- 12 Use of An Isolated Perfused Kidney to Assess Renal Clearance of Drugs: Information Obtained in Steady-State and Non-Steady-State Experimental Systems.- 13 Brain Microvessel Endothelial Cell Culture Systems.- 14 Methods to Study Drug Transport in Isolated Choroid Plexus Tissue and Cultured Cells.- 15 Brain Perfusion Systems for Studies of Drug Uptake and Metabolism in the Central Nervous System.- 16 In Vitro Nasal Models.- 17 Models for Investigation of Peptide and Protein Transport across Cultured Mammalian Respiratory Epithelial Barriers.- 18 Drug Transport across Xenopus Alveolar Epithelium in Vitro.- 19 In Situ and in Vivo Methods for Pulmonary Delivery.- 20 In Vitro Viable Skin Model.- 21 Isolated Perfused Porcine Skin Flap Systems.- 22 Vaginal Epithelial Models.- 23 Ocular Epithelial Models.