Micropropagation Methods in Temporary Immersion Systems

This volume presents vital techniques for the commercial micropropagation of plants. It explores numerous micropropagation protocols in different types of temporary immersion systems for plant species with agricultural, medicinal, ornamental, and forestry interest, detailing the type of bioreactor and the optimal parameters necessary to guarantee obtaining the largest number of commercial propagules. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. *

• Micropropagation Methods in Temporary Immersion Systems serves as an ideal guide to allow for the semi-automation and reduction of production costs during the micropropagation of plants.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Contenu

Temporary Immersion Systems in Plant Micropropagation.- Types of Temporary Immersion Systems Used in Commercial Plant Micropropagation.- Use of Temporary Immersion Systems in the Establishment of Biofactories.- Large-Scale Micropropagation of Vanilla ( Vanilla planifolia Jacks.) in a Temporary Immersion Bioreactor (TIB).- Temporary Immersion Bioreactors for Sugarcane Multiplication and Rooting.- Micropropagation of Stevia ( Stevia rebaudiana Bert.) in RITA ® .- In Vitro Multiplication of Agave ( A. marmorata and A. potatorum ) by Temporary Immersion in SETIS™ Bioreactor.- BioMINT: A Temporary Immersion System for Agave Micropropagation.- Plant Regeneration of Agave cupreata by Somatic Embryogenesis in a Temporary Immersion System with Silver Nanoparticles.- Micropropagation of Chayote ( Sechium edule L. ) var. virens levis in RITA ®.- Direct Shoot from Root and True-to-Type Micropropagation of Limonium 'Misty Blue' in Partially Immersed Culture on an Aluminum Mesh Raft.- Increased Multiplication Rates of Vriesea hieroglyfica (Carriere) E. Morren through a Temporary Immersion System.- Micropropagation of Encyclia cordigera (Kunth) Dressler in Ebb-and-Flow Bioreactor.- Micropropagation of Guarianthe skinneri (Bateman) Dressler & W. E. Higging in Temporary Immersion Bioreactors.- Alstroemeria Micropropagation in a RITA® Temporary Immersion System.- Proliferation of Axillary Shoots of Chestnut in Temporary Immersion Systems.- In Vitro Propagation of Plants via Organogenesis in Bambusa vulgaris Schrad. ex Wendl Using Temporary Immersion Systems.- Multiplication of Cupressus guadalupensis Using the RITA® Temporary Immersion System.- Secondary Embryogenesis of Linaloe in Temporary Immersion Bioreactors Type RITAÒ.- Temporary Immersion System for Biomass Production of Salvia spp: A Mini-Review.- Orchid Micropropagation Using Temporary Immersion Systems: A Review.- Conclusions and Perspectives on Plant Micropropagation in Temporary Immersion Systems.