Magnaporthe oryzae

This volume highlights molecular methods to study the phytopathogenic rice blast fungus Magnaporthe oryzae. Chapters in this book cover the history, development, and evolution of the pathogen; molecular methods to increase the knowledge of the biology, genetic, and metabolic diversity of the pathogen; and the pathogen's adaptability. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Magnaporthe oryzae: Methods and Protocols is a valuable resource for any scientist or researcher interested in learning more about this developing field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Contenu
The Impact of Blast Disease: Past, Present, and Future.- The Biology of Invasive Growth by the Rice Blast Fungus Magnaporthe oryzae.- Analysis of the Secondary Metabolism in Magnaporthe oryzae.- The Determination of Carbohydrates by High-Performance Anion-Exchange Chromatography Coupled with Pulsed Amperometric Detection (HPAEC-PAD).- Characterizing the Secretion Systems of Magnaporthe oryzae.- Specimen Preparation and Observations of Magnaporthe oryzae Appressorial Cells Under Electron Microscopy.- 4D Widefield Fluorescence Imaging of Appressorium Morphogenesis by Magnaporthe oryzae.- Tandem Affinity Purification (TAP) of Low Abundance Protein Complexes in Filamentous Fungi Demonstrated using Magnaporthe oryzae.- Quantitative Proteome and Phosphoproteome Profiling in Magnaporthe oryzae.- A Target-Based In Vivo Test System to Identify Novel Fungicides with Mode of Action in the HOG Pathway.- Making Visualization Work for You: Deriving Valuable Insights from Omics Data.- Genome Editing using CRISPR/Cas9 System in the Rice Blast Fungus.- Diurnal, Circadian, and Photomorphogenic Analyses in Magnaporthe oryzae.- Methods to Study Autophagocytosis in Magnaporthe oryzae.- Molecular Detection of Magnaporthe oryzae from Rice Seeds.- Isolation and Functional Analysis of Effector-Proteins of Magnaporthe oryzae.- Analysis of Genetic Variations and Genomic Instabilities in Magnaporthe oryzae.- Controllable Bypass Suppression in Magnaporthe oryzae.