Principles of Virology

Principles of Virology, the leading virology textbook in use, is an extremely valuable and highly informative presentation of virology at the interface of modern cell biology and immunology. This text utilizes a uniquely rational approach by highlighting common principles and processes across all viruses. Using a set of representative viruses to illustrate the breadth of viral complexity, students are able to understand viral reproduction and pathogenesis and are equipped with the necessary tools for future encounters with new or understudied viruses.

This fifth edition was updated to keep pace with the ever-changing field of virology. In addition to the beloved full-color illustrations, video interviews with leading scientists, movies, and links to exciting blogposts on relevant topics, this edition includes study questions and active learning puzzles in each chapter, as well as short descriptions regarding the key messages of references of special interest.

Volume I: Molecular Biology focuses on the molecular processes of viral reproduction, from entry through release. Volume II: Pathogenesis and Control addresses the interplay between viruses and their host organisms, on both the micro- and macroscale, including chapters on public health, the immune response, vaccines and other antiviral strategies, viral evolution, and a brand new chapter on the therapeutic uses of viruses. These two volumes can be used for separate courses or together in a single course. Each includes a unique appendix, glossary, and links to internet resources.

Principles of Virology, Fifth Edition, is ideal for teaching the strategies by which all viruses reproduce, spread within a host, and are maintained within populations. This edition carefully reflects the results of extensive vetting and feedback received from course instructors and students, making this renowned textbook even more appropriate for undergraduate and graduate courses in virology, microbiology, and infectious diseases.

Auteur

Jane Flint is Professor Emerita of Molecular Biology at Princeton University. Dr. Flint's research focused on investigation of the mechanisms by which viral gene products modulate host pathways and antiviral defenses to allow efficient reproduction in normal human cells of adenoviruses, viruses that are used in such therapeutic applications as gene transfer and cancer treatment.

Vincent R. Racaniello is Higgins Professor of Microbiology & Immunology at Columbia University Vagelos College of Physicians & Surgeons. Dr. Racaniello has been studying viruses for over 40 years, including polio- virus, rhinovirus, enteroviruses, hepatitis C virus, and Zika virus. He blogs about virus-es at virology.ws and is host of This Week in Virology.

Glenn F. Rall is a Professor and the Chief Academic Officer at the Fox Chase Cancer Center, and is an Adjunct Professor in the Microbiology and Immunology departments at the University of Pennsylvania, as well as Thomas Jefferson, Drexel, and Temple Universities. Dr. Rall studies viral infections of the brain and the immune responses to those infections, with the goal of defining how viruses contribute to disease.

Theodora Hatziioannou is a Research Associate Professor at Rockefeller University and is actively involved in teaching programs at Albert Einstein College of Medicine. Dr. Hatziioannou has worked on multiple viruses with a focus on retroviruses and the molecular mechanisms that govern virus tropism and on the improvement of animal models for human disease.

Anna Marie Skalka is a Professor Emerita and former Senior Vice President for Basic Research at the Fox Chase Cancer Center. Dr. Skalka is internationally recognized for her contributions to the understanding of the biochemical mechanisms by which retroviruses replicate and insert their genetic material into the host genome, as well as her research into other molecular aspects of retrovirus biology.

Contenu

Volume 1
Preface

Acknowledgments

About the Authors

PART I: The Science of Virology

1 Foundations

Luria's Credo

Viruses Defined

Why We Study Viruses

Viruses Are Everywhere

Viruses Infect All Living Things

Viruses Can Cause Human Disease

Viruses Can Be Beneficial

Viruses "R" Us

Viruses Can Cross Species Boundaries

Viruses Are Unique Tools To Study Biology

Virus Prehistory

Viral Infections in Antiquity

The First Vaccines Microorganisms as Pathogenic Agents

Discovery of Viruses

The Defining Properties of Viruses

The Structural Simplicity of Virus Particles

The Intracellular Parasitism of Viruses

Cataloging Animal Viruses

The Classical System

Classification by Genome Type: the Baltimore System

A Common Strategy for Viral Propagation

Perspectives

References

Study Questions

2 The Infectious Cycle

Introduction

The Infectious Cycle

The Cell

Entering Cells

Viral RNA Synthesis

Viral Protein Synthesis

Viral Genome Replication

Assembly of Progeny Virus Particles

Viral Pathogenesis

Overcoming Host Defenses

Cultivation of Viruses

Cell Culture

Embryonated Eggs

Laboratory Animals

Assay of Viruses

Measurement of Infectious Units

Efficiency of Plating

Measurement of Virus Particles

Viral Reproduction: the Burst Concept

The One-Step Growth Cycle

One-Step Growth Analysis: a Valuable Tool for Studying Animal Viruses

Global Analysis

DNA Microarrays

Mass Spectrometry

Protein-Protein Interactions

Single-Cell Virology

Perspectives

References

Study Questions

PART II: Molecular Biology

3 Genomes and Genetics

Introduction

Genome Principles and the Baltimore System

Structure and Complexity of Viral Genomes

DNA Genomes

RNA Genomes

What Do Viral Genomes Look Like?

Coding Strategies

What Can Viral Sequences Tell Us?

The "Big and Small" of Viral Genomes: Does Size Matter?

The Origin of Viral Genomes

Genetic Analysis of Viruses

Classical Genetic Methods

Engineering Mutations into Viral Genomes

Engineering Viral Genomes: Viral Vectors

Perspectives

References

Study Questions

4 Structure

Introduction

Functions of the Virion

Nomenclature

Methods for Studying Virus Structure

Building a Protective Coat

Helical Structures

Capsids with Icosahedral Symmetry

Other Capsid Architectures

Packaging the Nucleic Acid Genome

Direct Contact of the Genome with a Protein Shell

Packaging by Specialized Viral Proteins

Packaging by Cellular Proteins

Viruses with Envelopes

Viral Envelope Components

Simple Enveloped Viruses: Direct Contact of External Proteins with the Capsid or Nucleocapsid

Enveloped Viruses with an Additional Protein Layer

Large Viruses with Multiple

Structural Elements Particles with Helical or Icosahedral Parts Alternative Architectures

Other Components of Virions

Enzymes

Other Viral Proteins

Cellular Macromolecules

Mechanical Properties of Virus Particles

Investigation of Mechanical Properties of Virus Particles

Stabilization and Destabilization of Virus Particles

Perspectives

References

Study Questions

5 Attachment and Entry

Introduction

Attachment of Virus Particles to Cells

General Principles

Identification of Receptors for Virus Particles

Virus-Receptor Interactions

Entry into Cells

Virus-induced Signaling via Cell Receptors

Routes of entry

Membrane Fusion

Intracellular Trafficking and Uncoating

Movement of Viral and Subviral Particles within Cells

Uncoating of enveloped viruses

Uncoating of non-enveloped viruses

Import of Viral Genomes into the Nucleus

The Nuclear Pore Complex

Nuclear Localization Signals

Import of RNA Genomes

Import of DNA Genomes

Import of Retroviral Genomes

Perspectives

References

Study Questions

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