Isothermal Titration Calorimetry in Enzymology

Auteur
Associate Professor Anthony Mittermaier works in the Department of Chemistry, McGill University, Montreal, Canada. Justin Di Trani is a Postdoctoral Fellow at The Hospital for Sick Children, Molecular Medicine Program, Toronto, Canada.

Texte du rabat
Isothermal Titration Calorimetry in Enzymology: Techniques and Applications provides a thorough, practical overview of ITC as a productive and powerful tool for quantifying enzyme catalysis and interactions with substrates, products, cofactors, and inhibitors. Here, global experts in the methodology discuss the full capabilities of ITC and how it may be applied in research, medicine, and industry and offer a basis to continue leveraging ITC as its uses expand and evolve. This book bridges the gap between research and application by giving extensive technical details on cutting edge ITC techniques across a wide swath of both ITC kinetics measurements and ITC thermodynamic measurements, alongside case studies illustrating the depth of knowledge that can be obtained. Applications discussed include measuring binding kinetics by ITC, analyzing ITC kinetic data, in vivo enzyme kinetics by ITC, analyzing enzyme kinetics in crowded solutions, studying catalysis of biological substrates, interpreting ITC binding curves, ITC in drug discovery, ITC in the development of cancer therapeutics, ITC analysis of aminoglycoside resistance enzymes, studying enzyme interactions with toxic metals, using ITC to study ubiquitination, and ITC studies of glycoside hydrolases, among others.

Contenu

PART 1: ITC kinetics measurements 1. Enzyme kinetics by ITC 2. Measuring binding kinetics by ITC 3. Analysing ITC kinetic data with AFFinimeter software 4. In vivo enzyme kinetics by ITC 5. Characterizing enzyme kinetics by 2D-ITC 6. Measuring enzyme stability using ITC 7. Kinetics of immobilized enzymes 8. Enzyme kinetics in crowded solutions 9. Kinetics of nanozymes 10. Using ITC to characterize enzyme allostery 11. Signal amplification in ITC kinetics experiments 12. ITC kinetic studies of 6-phytase 13. Catalysis of biological substrates of nuclear metallohydrolases 14. Alpha-amylase activity and inhibition 15. Enzyme stability and activity of psycrophillic enzymes 16. Non-MM kinetics of peroxidases and application to industrial wastes 17. Kinetics of diphosphohydrolases PART 2: ITC thermodynamic measurements 18. Interpretation of ITC binding curves 19. Analysis of ITC binding data using SEDPHAT 20. ITC in drug discovery 21. ITC in the development of cancer therapeutics 22. Mechanisms of aminoglycoside resistance enzymes 23. Using ITC to characterize the interactions of natural products with biomolecules 24. ITC studies of metalloenzymes 25. Enzyme interactions with toxic metals 26. Thermodynamics of molecular machines 27. Thermodynamics of protein chaperones 28. Characterizing protein interactions in apoptosis by ITC 29. Using ITC to study ubiquitination 30. Thermodynamics of transition state analogs 31. Thermodynamics of recombinant enzymes in biotechnology 32. Ion Binding to Transport Proteins using ITC 33. Urease from Helicobacter pylori, lessons from ITC 34. Substrate recognition by Chitinases 35. ITC studies of glycoside hydrolases