Intercellular Signalling in the Mammary Gland

All being done, we went to Mrs Shipmans, who is a great butter-woman; and I did see there the most of milke and cream, and the cleanest, that I ever saw in my life (29 May 1661). Among others, Sir Wm. Petty did tell me that in good earnest, he hath in his will left such parts of his estate to him that could invent such and such things -as among others, that could discover truly the way of milk coming into the breasts of a woman ... (22 March 1665). My wife tells me that she hears that my poor aunt James hath had her breast cut off here in tow- her breast having long been out of order (5 May 1665). From the Diary of Samuel Pepys, published as The Shorter Pepys (edited by R. Latham), Penguin Books (1987) The long-standing ultimate importance of research on the mammary gland is illustrated by the importance attached to cows' milk for human consumption, to human lactation and to breast cancer by Samuel Pepys and his contemporaries in the middle of the 17th century. Research has tended to develop in isolation in these three areas of continuing contemporary importance largely because in most countries, the underlying science of agricultural productivity is funded separately from the underlying science of human health and welfare.

Contenu
Mammary Development: Growth and Growth Factors.- The environment of the mammary secretory cell.- Role of bovine placental lactogen in intercellular signalling during mammary growth and lactation.- Expression of Hox genes in normal and neoplastic mouse mammary gland.- Local signals for growth cessation and differentiation in the mammary gland.- Apoptosis in mammary gland involution: isolation and characterization of apoptosis-specific genes.- The role of erbB-family genes and Wnt genes in normal and preneoplastic mammary epithelium, studied by tissue reconstitution.- VerbB induces abnormal patterns of growth in mammary epithelium.- Interplay between epidermal growth factor receptor and estrogen receptor in progression to hormone independence of human breast cancer.- The effect of plane of nutrition on mammary gland development in prepubertal goats.- Activation of basic fibroblast growth factor (bFGF) by heparan sulphate (HS).- High density culture of immuno-magnetically separated human mammary luminal cells.- Molecular analysis of the effects of oestradiol in an in vivo model of normal human breast proliferation.- Juxtacrine growth stimulation of mouse mammary cells in culture.- Fibroblast growth factors in mammary development and cancer.- Evidence of growth factor production by sheep myoepithelial and alveolar epithelial cells: potential for autocrine/paracrine interactions.- Derivation of conditionally immortal mammary cell lines.- Effect of relaxin on primary cultures of goat mammary epithelial cells.- Differential effects of growth factors acting by autocrine and paracrine pathways in breast cancer cells.- Validation of transforming growth factor ß1 binding assay for bovine mammary tissue.- Endocrine effect of IGF-I on mammary growth in prepubertal heifers.- Localcontrol of mammary apoptosis by milk stasis.- Immunolocalisation of insulin-like growth factors and basic fibroblast growth factor in the bovine mammary gland.- Flow sorting and clonal analysis of mouse mammary epithelium.- Cytokine expression in normal and neoplastic breast tissue.- Production of an insulin-like growth factor binding protein by the involuting rat mammary gland.- Developmental and hormonal regulation of Wnt gene expression in the mouse mammary gland.- Mammary Development: Differentiation and Gene Expression.- Extracellular matrix dependent gene regulation in mammary epithelial cells.- ?-lactalbumin regulation and its role in lactation.- Regulation of milk secretion and composition by growth hormone and prolactin.- Basement membrane in the control of mammary gland function.- Asynchronous concurrent secretion of milk proteins in the tammar wallaby (Macropus eugenii).- Heterogeneous expression and synthesis of human serum albumin in the mammary gland of transgenic mice.- Post-transcriptional regulation of casein gene expression by prolactin and growth hormone in lactating rat mammary gland.- Characterisation of transcriptional control elements in the mammary gland and pancreas: studies of the carboxyl ester lipase gene.- Oligosaccharide analysis of epithelial mammary tissues.- Human bile salt-activated lipase: structural organisation at the c-terminus.- Dramatic changes in gap junction expression in the mammary gland during pregnancy, lactation and involution.- Aromatase induction in stromal vascular cells from human breast adipose tissue by growth factors.- Developmental regulation of homeobox genes in the mouse mammary gland.- Murine ?-lactalbumin gene inactivation and replacement.- Differential expression of ß-lactoglobulin alleles A and B in dairycattle.- DNA replication, ß-casein gene transcription and DNase-I sensitivity of ß-casein gene in rabbit mammary cell nuclei.- Milk Secretion.- Autocrine control of milk secretion: development of the concept.- Endocrine and autocrine strategies for the control of lactation in women and sows.- Autocrine control of milk secretion: from concept to application.- A role for medium-chain fatty acids in the regulation of lipid synthesis in milk stasis?.- The secretory pathway for milk protein secretion and its regulation.- In vitro effects of oxytocin and ionomycin on lipid secretion by rat mammary gland. Role of the myoepithelial cells.- Dose-dependent effects of oxytocin on the microcirculation in the mammary gland of the lactating rat.- Effects of triiodothyronine administration on the disposal of oral [1-14C]-triolein, lipoprotein lipase activity and lipogenesis in the rat during lactation and on removal of the litter.- Induction of immune responses in the bovine mammary gland.- Cultured mammary epithelial cells secrete a factor which suppresses lipoprotein lipase production by 3T3-L1 adipocytes.- Localisation of annexin V and annexin VI in lactating mammary epithelial cells.- Appendix I.- Scientific Papers published since 1984 by speakers at this Symposium in the CAB abstracts database.- Contributors.