Insect Cell Cultures

Insect Cell Cultures: Fundamental and Applied Aspects is a comprehensive reference work, covering the key issues involved in nearly 30 review papers: cell lines (development, characterisation, physiology, cultivation and medium design); viruses (virus-cell interactions, replication, recombinant construction, infection kinetics, post-translational modification and passage effects); engineering (shear, bioreactors including perfusion, immobilisation, scale-up and modelling, downstream processing); applications; economics and regulatory aspects. For cell biologists, biochemists, molecular biologists, virologists, immunologists and other basic and applied disciplines related to cell culture engineering, both academic and industrial.

Résumé
A reference work, covering the issues involved in nearly 30 review papers: cell lines; viruses; engineering; applications; economics and regulatory aspects. It is intended for cell biologists, biochemists, molecular biologists, virologists, immunologists and other basic and applied disciplines related to cell culture engineering.

Contenu
Insect Cell Lines.- Development and characterization of insect cell lines.- New approaches to insect tissue culture.- Transgenic insect cells: mosquito cell mutants and the dihydrofolate reductase gene.- Insect cell physiology.- Insect cell cultivation: growth and kinetics.- Medium design for insect cell culture.- Viruses.- Baculovirus-insect cell interactions.- Replication patterns and cytopathology of cells infected with baculoviruses.- Construction of baculovirus recombinants.- Passage effect of virus infection in insect cells.- Post-translational modifications in insect cells.- Productivity of insect cells for recombinant proteins.- Chaperone and foldase coexpression in the baculovirus-insect cell expression system.- Engineering Aspects.- Shear sensitivity of insect cells.- Insect cell bioreactors.- Perfusion bioreactors for the production of recombinant proteins in insect cells.- Immobilization of insect cells.- Modelling baculovirus infection of insect cells in culture.- Scale up aspects of sparged insect-cell bioreactors.- Oxygen gradients in small and big sparged insect-cell bioreactors.- Downstream processing of insect cell cultures.- Applications.- Parvovirus diagnostics and vaccine production in insect cells.- Classical swine fever virus diagnostics and vaccine production in insect cells.- Production of multidomain complement glycoproteins in insect cells.- Economic and Regulatory Aspects.- Economics of baculovirus-insect cell production systems.- Safety aspects of insect cell culture.- Regulatory issues in the use of insect-cell culture.