Immunology in Plant Sciences

Modem Methods of Plant Analysis When the handbook Modern Methods of Plant Analysis was fIrst introduced in 1954 the considerations were: 1. the dependence of scientifIc progress in biology on the improvement of existing and the introduction of new methods; 2. the difficulty in fInding many new analytical methods in specialized journals which are normally not accessible to experimental plant biologists; 3. the fact that in the methods sections of papers the description of methods is frequently so compact, or even sometimes so incomplete that it is difficult to reproduce experiments. These considerations still stand today. The series was hIghly successful, seven volumes appearing between 1956 and 1964. Since there is still today a demand for the old series, the publisher has decided to resume publication of Modern Methods of Plant Analysis. It is hoped that the New Series will be just as acceptable to those working in plant sciences and related fIelds as the early volumes undoubtedly were. It is difficult to single out the major reasons for success of any publication, but we believe that the methods published in the fIrst series were up-to-date at the time and presented in a way that made description, as applied to plant material, complete in itself with little need to consult other publications. Contributing authors have attempted to follow these guidelines in this New Series of volumes.

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With contributions by numerous experts

Contenu
Plant Hormone Immunoassays Based on Monoclonal and Polyclonal Antibodies.- 1 Introduction.- 2 Synthesis of Immunogens.- 3 Radio- and Enzyme-Labeling of Plant Hormones.- 4 Immunological Procedures.- 5 Antiserum Processing.- 6 Production of Monoclonal Antibodies.- 7 Radioimmunoassay Procedures.- 8 Enzyme Immunoassays Based on Monoclonal Antibodies.- 9 Plant Immunoanalysis.- References.- Radioimmunoassay and Gas Chromatography/Mass Spectrometry for Cytokinin Determination.- 1 Introduction.- 2 Theoretical Considerations for Analytical Methods.- 3 Radioimmunoassay.- 4 Gas Chromatography/Mass Spectrometry.- 5 Concluding Remarks.- References.- Immunodetection of Phytochrome: Immunocytochemistry, Immunoblotting, and Immunoquantitation.- 1 Introduction.- 2 Immunocytochemistry.- 3 Immunoblotting.- 4 Immunoquantitation.- 5 Concluding Remarks.- References.- Radioimmunoassay for a Soybean Phytoalexin.- 1 Isolation of Glyceollin I.- 2 Synthesis of Glyceollin I-BSA Conjugate.- 3 Generation of Antisera.- 4 Preparation of the [125I]-Glyceollin I Tracer.- 5 Radioimmunoassay for Glyceollin I.- 6 Specificity of the Radioimmunoassay.- 7 Application of the Radioimmunoassay for Glyceollin I to the Quantitation of Phytoalexins in Infected Soybean Tissue.- References.- The Measurement of Low-Molecular-Weight, Non-Immunogenic Compounds by Immunoassay.- 1 Introduction.- 2 Development of an Immunoassay.- 3 Immmunoassays.- Appendix: Sources of Materials and Equipment for ELISA.- References.- Radioimmunoassay and Western Blot Analysis of Acyl Carrier Protein Isoforms in Plants.- 1 Introduction.- 2 Production of ACP Antibody.- 3 Radioimmunoassay.- 4 Immunological Analysis of ACP Isoforms.- 5 Conclusion.- References.- Immunofluorescent Labelling of Enzymes.- 1 Introduction.- 2 General Methodology.- 2.5 Controls.- 3 Examples for Localization of Enzymes in Plant Tissue by Immunofluorescent Labeling.- References.- Quantitative Immunochemistry of Plant Phosphoenolpyruvate Carboxylases.- 1 Purificationof PEPC Preparation of Immune Sera.- 2 Quantitative Immunoprecipitation in Gels.- 3 Immunotitration Coupled to Enzyme Activity.- 4 Quantitative Immunoprecipitation in Extracts.- 5 Immunoaffinity Chromatography.- 6 Concluding Remarks.- References.- Immunochemical Methods for Higher Plant Nitrate Reductase.- 1 Introduction.- 2 Nitrate Reductase Purification.- 3 Production of Nitrate Reductase Antiserum.- 4 Characterization of Nitrate Reductase Antiserum.- 5 Applications.- 6 Summary.- References.- Immunological-Cytochenlical Localization of Cell Products in Plant Tissue Culture.- 1 Introduction.- 2 The Laticifer.- 3 The Tissue Culture System for the Asclepiadaceae.- 4 Future Uses of Immunological-Cytochemical Techniques in Tissue Culture.- References.- Measurement of Oat Globulin by Radioimmunoassay.- 1 Introduction.- 2 Requirements for the Radioimmunoassay.- 3 Designing the RIA.- 4 Summary.- References.- Immunocytocheniistry of Chloroplast Antigens.- 1 Introduction.- 2 Light Microscopy.- 3 Electron Microscopy.- References.