Studying Cell Metabolism and Cell Interactions Using Microfluidic Devices Coupled with Mass Spectrometry

This thesis describes a new approach for cell analysis by the rapid developing microfluidic technology. The nominee has made great contributions to develop a new analysis platform which combined microfluidic devices with mass spectrometry to determine the trace compounds secreted by cells. Based on this analysis platform, she studied the specific cell secreting behaviors under controlled microenvironment, of which the secretion compounds were qualified and semi-quantified by mass spectrometry. A novel cell sorting device integrated homogenous porous PDMS membrane was invented to classify cells from real samples based on the size difference. The nominee further studied the signal transmission between different cells, and the signal chemicals were qualitative and quantitative monitored by the analysis platform. This indicates the potential significant application of the new cell analysis platform in medicine screening and early diagnosis.

Describes a new cell analysis platform which combines microfluidic device with mass spectrometry to determine trace chemicals secreted by the cells Develops microfluidic devices integrating the functions of cell culture, drug stimulation, and secretion pretreatment Invents an energy-saving cell sorting microluidic device, which successfully achieved a separation efficiency of 97% Establishes a cell co-culture microfluidic device to study the signal transmission functions between related organisms Nominated as an outstanding contribution by Department of Chemistry, Tsinghua University

Contenu
Introduction.- Analysis of Herbicides on a Single C30 Bead via the Platform Combined Microfluidic Device with ESI-Q-TOF-MS.- Monitoring of Glutamate Release from Neuronal Cell Based on the Analysis Platform Combining the Microfluidic Devices with ESI-Q-TOF-MS.- Microfluidic Device with Integrated Porous Membrane for Cell Sorting and Separation.- Cell Co-culture and Signaling Analysis Based on Microfluidic Devices Coupling with ESI-Q-TOF-MS.