High Performance Liquid Chromatography in Plant Sciences

Modern Methods of Plant Analysis When the handbook Modern Methods of Plant Analysis was first introduced in 1954 the considerations were: 1. the dependence of scientific progress in biology on the improvement of existing and the introduction of new methods; 2. the difficulty in finding many new analytical methods in specialized journals which are normally not accessible to experimental plant biologists; 3. the fact that in the methods sections of papers the description of methods is frequently so compact, or even sometimes so incomplete that it is difficult to reproduce experiments. These considerations still stand today. The series was highly successful, seven volumes appearing between 1956 and 1964. Since there is still today a demand for the old series, the publisher has decided to resume publication of Modern Methods of Plant Analysis. It is hoped that the New Series will be just as acceptable to those working in plant sciences and related fields as the early volumes undoubtedly were. It is difficult to single out the major reasons for success of any publication, but we believe that the methods published in the first series were up-to-date at the time and presented in a way that made description, as applied to plant material, complete in itselfwith little need to consult other publications. Contributing authors have attempted to follow these guidelines in this New Series of volumes.

Texte du rabat

With contributions by numerous experts

Contenu
High Performance Liquid Chromatography of Gibberellins.- 1 Introduction.- 2 Extraction and Purification.- 3 Group Separation Procedures.- 4 High Performance Liquid Chromatography (HPLC).- 5 Recent Developments and Prospects.- References.- Characterization of Cytokinins and Related Compounds by HPLC.- 1 Introduction.- 2 Cytokinin Isolation and Sample Preparation.- 3 Preparation of Mobile Phase.- 4 Chromatography.- 5 Concluding Remarks.- References.- Separation and Purification of Abscisic Acid and Its Catabolites by High Performance Liquid Chromatography.- 1 Introduction.- 2 Abscisic Acid and Its Catabolites.- 3 Use of High Performance Liquid Chromatography to Characterize Abscisic Acid and Its Catabolites.- 4 Concluding Remarks.- References.- The Determination of Abscisic Acid by High Performance Liquid Chromatography.- 1 Introduction.- References.- High Performance Liquid Chromatography and the Analysis of Indole-3-Acetic Acid, and Some of Its Decarboxylated Catabolites in Scots Pine (Pinus sylvestris L.).- 1 Introduction.- 2 Internal Standardization.- 3 Extraction and Purification.- 4 High Performance Liquid Chromatography.- 5 Examples.- 6 Conclusions.- References.- HPLC for the Separation and Determination of Phenolic Compounds in Plant Cell Walls.- 1 Introduction.- 2 General Considerations.- 3 The Recommended Procedure Isolation of Cell Walls.- 4 The Recommended Procedure Release of Phenolic Acids and Aldehydes from Cell Walls and Their Separation and Determination by HPLC (C18 Reverse Phase) (Methods 1 and 2).- 5 Alternative Procedure Release of Phenolic Acids and Aldehydes from Cell Walls and Their Separation and Determination by HPLC (PRP-1 Resin) (Methods 3 and 4).- 6 Amount of Phenolic Acids and Aldehydes Released from the Cell Walls of GraminaceousPlants.- References.- HPLC of Thiophenes for Phytochemical and Biochemical Research.- 1 Introduction.- 2 Material and Methods.- 3 Use of HPLC for Phytochemical Research on Thiophenes.- 4 Use of HPLC for Enzymatic Research on Thiophenes.- 5 Conclusions.- References.- High Performance Liquid Chromatography of Ascorbic Acid.- 1 Introduction.- 2 Sample Preparation.- 3 Stationary and Mobile Phases.- 4 Detection Techniques for Ascorbic Acid.- References.- High Performance Liquid Chromatography of Phytoalexins.- 1 Introduction.- 2 Instrumentation.- 3 Preparative HPLC of Phytoalexins.- 4 Analytical HPLC of Phytoalexins.- 5 Application of HPLC to the Solution of Outstanding Problems in Phytoalexin Research.- 6 Concluding Remarks.- References.- Analysis of Lipids by High Performance Liquid Chromatography.- 1 Introduction.- 2 Instrumentation.- 3 Lipid HPLC Applications.- References.- Practical High Performance Liquid Chromatography of Proteins and Peptides.- 1 Introduction.- 2 Size Exclusion HPLC.- 3 Ion Exchange HPLC.- 4 Reversed Phase HPLC.- 5 Multidimensional HPLC.- References.- Monitoring Polyamines in Plant Tissues by High Performance Liquid Chromatography.- 1 Introduction.- 2 High Performance Liquid Chromatography of Dansylamines.- 3 High Performance Liquid Chromatography of Benzoylamines.- 4 Ion-Pair Reverse Phase Chromatography of Underivatized Polyamines.- 5 Ion-Exchange Chromatography of Underivatized Polyamines.- 6 Conclusion.- References.- Analysis of Alkaloids in Tobacco Callus by HPLC.- 1 Introduction.- 2 Experimental.- 3 Results and Discussion.- 4 Conclusions.- References.