Parameter estimation of Michaelis-Menten kinetics

The main objective of this work was to investigate the application of experimental design techniques for the identification of Michaelis-Menten kinetic parameters. More specifically, this study attempts to elucidate the relative advantages/disadvantages of employing complex experimental design techniques in relation to equidistant sampling when applied to different reactor operation modes. All studies were supported by simulation data of a generic enzymatic process that obeys to the Michaelis-Menten kinetic equation. Different aspects were investigated, such as the influence of different reactor operation modes (batch, fed-batch with pulsewise feeding and fed- batch with continuous feeding) and the experimental design optimality criteria on the effectiveness of kinetic parameters identification.

Auteur

Filipe Ataíde - Ph.D. student at Universidade Nova de Lisboa (Portugal), M.Sc. in Chemical and Biochemical Eng. (2007). Research areas: Mass transfer, modeling and nanofluids. Bernd Hitzmann - Professor at the Leibniz University of Hannover (Germany). Research areas: Bioprocess monitoring, modeling, optimization and automation.