Environmental Toxicology and Toxicogenomics

This detailed book provides an accessible compendium of up-to-date methods in the fields of environmental toxicology, molecular toxicology, and toxicogenomics. Organized into four major sections, the volume examines methods utilizing model animal species, such as nematode, fruit fly, mice, chicken, and amphibians, methods using plants to study chemical toxicity, applying the Ames assay to chemical mutagenicity study, as well as methods for environmental chemical analysis. Although this book is divided into these parts, the methods can be used across species. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Environmental Toxicology and Toxicogenomics: Principles, Methods, and Applications serves as a valuable resource for the scientific community, particularly for young scientists and graduate and undergraduate students, inspiring more research in the vitally important field of environmental toxicity, molecular toxicology, and toxicogenomics.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Contenu

Detection of Caenorhabditis elegans Germ Cell Apoptosis Following Exposure to Environmental Contaminant Mixtures: A Crude Oil-Dispersant Mixture Example.- High-Throughput Measurement for Toxic Effects of Metal Mixtures in Caenorhabditis elegans .- Evaluations of Environmental Pollutants-Induced Mitochondrial Toxicity Using Caenorhabditis elegans as a Model System.- Methods to Assay the Behavior of Drosophila melanogaster for Toxicity Study.- Investigating the Joint Effects of Pesticides and Ultraviolet-B Radiation in Xenopus laevis and Other Amphibians.- Identification of Stable Reference Genes for Toxicogenomic and Gene Expression Analysis.- Semi-Quantitative RT-PCR: An Effective Method to Explore the Regulation of Gene Transcription Level Affected by Environmental Pollutants.- Employing Multiple New Neurobiological Methods to Investigate Environmental Neurotoxicology in Mice.- Analyses of Epigenetic Modification in Environmental Pollutants-InducedNeurotoxicity.- The Application of Omics Technologies in the Research of Neurotoxicology.- Flow Cytofluorometric Analysis of Molecular Mechanisms of Premature Red Blood Cell Death.- Practical Methods and Technologies in Environmental Epidemiology.- In Ovo Early-in-Life Inhalation Exposure to Gas/Aerosol with a Chicken Embryo Model.- DNA Damage in Liver Cells of the Tilapia Fish Oreochromis mossambicus Larva Induced by the Insecticide Cyantraniliprole at Sublethal Doses During Chronic Exposure.- Impact of Nanoparticles on Plant Growth, Development, and Biomass.- Biochemical and Physiological Toxicity of Nanoparticles in Plant.- Determination of Oxidative Stress and Antioxidant Enzyme Activity for Physiological Phenotyping During Heavy Metal Exposure.- Comprehensive Phytotoxicity Assessment Protocol for Engineered Nanomaterials.- Detection of Cadmium Toxicity in Plant.- Mutagenicity Evaluation of Nanoparticles by the Ames Assay.- Dispersive Solid Phase Extraction of Multiresidue Pesticides in Food and Water Samples.- Quantitative Analysis of Multiresidue Pesticides Using Gas Chromatography/Mass Spectrometry.- Determination of the N-Nitroso Compounds in Mouse Following RDX Exposure.- Determination of Metal Content in Drosophila melanogaster During Metal Exposure.- Microplastics: A Review of Methodology for Sampling and Characterizing Environmental and Biological Samples.