Circadian Clocks

This volume provides backgrounds, methods, and troubleshooting for the analysis of circadian rhythms at the molecular, cellular, and organismal levels in vertebrates and invertebrates, and also covers light input, sleep, circalunar rhythms, and seasonal rhythms. The chapters in this book discuss behavioral and biological testing, histochemistry, single-cell imaging, electrophysiology, photochemistry, genetics, genomics, transcriptomics, proteomics, metabolomics, systems biology, chemical biology, and mathematical modeling. In the Neuromethods series style, chapters include experimental detail and key advice from the specialists needed to successfully carry out these methods in your laboratory.

Cutting-edge and practical, Circadian Clocks is a valuable resource for all researchers seeking to learn about established and novel methods in chronobiology, and the molecular mechanisms of the rhythmic control of physiology and behavior.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Contenu
Circadian Rhythm Measurements in Humans.- Biological Rhythm Measurements in Rodents.- Assaying Circadian Locomotor Activity Rhythm in Drosophila.- Neuroanatomy of the Suprachiasmatic Nucleus (SCN); Multicolor Immunohistochemistry and 3D Reconstruction.- Long-Term Imaging and Electrophysiology of Single Suprachiasmatic Nucleus Neurons.- Patch-Clamp Electrophysiological Analysis of Murine Melanopsin Neurons.- Intrinsically-Photosensitive Retinal Ganglion Cells: Assessment of their Influence on Pupillary Responses in Primates.- Spectroscopic Analysis of Wavelength Sensitivities of Opsin-Type Photoreceptor Proteins.- Nocturnal and Diurnal Behavior Assessed by the 'Work-for-Food' Protocol in Small Rodents.- Assessment of Circalunar (~Monthly) Rhythms.- Identification and Characterization of Genes Involved in Vertebrate Photoperiodism.- Methods to Study Sleep in Zebrafish.- Assessment of Sleep in Mice.- Design Principles and Analysis Guidelines for Understanding Time-of-Day Effects in the Brain.- Proteomics Approaches to Assess Sleep and Circadian Rhythms.- A Primer for Circadian Metabolic Profile Analysis Using Multi-Platform Metabolomics.- Next-Generation Mice Genetics for Circadian Studies.- Chronophotopharmacology: Methodology for High Spatiotemporal Control Over the Circadian Rhythm with Light.- Mathematical Modeling of Circadiam Rhythms.