Capillary Electrophoresis of Biomolecules

This book details key techniques used to investigate Capillary electrophoresis (CE). It focuses on simple and complex carbohydrates (polysaccharides), aminoacids, peptides and proteins, enzymes, and nucleic acids.

Capillary electrophoresis (CE) is a relatively new separation technique suitable for handling small amounts of sample very important in bioanalytical research and in various clinical, diagnostic, genetic, and forensic applications. In Capillary Electrophoresis of Biomolecules: Methods and Protocols, expert researchers in the field provide key techniques to investigate CE focusing on simple and complex carbohydrates (polysaccharides), aminoacids, peptides and proteins, enzymes, and nucleic acids. Along with practical procedures, reviews discussing CE applications related to bio(macro)molecules are also included. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Capillary Electrophoresis of Biomolecules: Methods and Protocols provides the reader with the latest break throughs and improvements in CE and CE techniques applied to several classes of bio(macro)molecules.

Focuses on experimental methods for the characterization of carbohydrates, mono-, oligo-, and polysaccharides Presents current, up-to-date methodologies Features tips on troubleshooting and avoiding known pitfalls Includes supplementary material: sn.pub/extras

Contenu

Recent Advances in Capillary Electrophoresis-Based Proteomic Techniques for Biomarker Discovery.- Capillary Electrophoresis-mass Spectrometry of Carbohydrates.- Approaches to Enhancing the Sensitivyty of Carbohydrate Separations in Capillary Electrophoresis.- Determination of Monosaccharides Derivatized with 2-Aminobenzoic Acid by Capillary Electrophoresis.- Determination of Mono- Di- and Oligosaccharides by Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection.- Separation of Chitooligosaccharides in Acidic Solution by Capillary Electrophoresis.- Capillary Electrophoresis for the Analysis of Glycosaminoglycan-derived Disaccharides.- High Throughput Capillary Electrophoresis Mass Spectrometry: From Analysis of Amino Acids to Analysis of Protein Complexes.- Separation of Amino Acids by Capillary Electrophoresis with Light-emitting Diode-induced Fluorescence in the Presence of Electroosmotic Flow.- Quantification of Arginine and Dimethylated Arginines in Human Plasma by Field-amplified Sample Injection Capillary Electrophoresis UV Detection.- Capillary Electrophoresis-Mass Spectrometry for Peptide Analysis: Target-Based Approaches and Proteomics/Peptidomics Strategies.- High Resolution Proteome/peptidome Analysis of Body Fluids by Capillary Electrophoresis Coupled with MS.- Contribution of CE to the Analysis of Protein or Peptide Biomarkers.- Highly Charged Polyelectrolyte Coatings to Prevent Adsorption During Protein and Peptide Analysis in Capillary Electrophoresis.- Capillary Electrophoresis with Laser Induced Fluorescence Detection of Proteins from Two Types of Complex Sample Matrices: Food and Biological Fluids.- Automated Capillary Electrophoresis in the Screening for Haemoglobinopathies.- Protein Fingerprinting of Staphylococcus aureus by Capillary Electrophoresis with On-capillary Derivatization and Laser-induced Fluorescence Detection.- Capillary Electrophoresis of Seed Storage Proteins: The Separation and Identification of Microheterogeneous Rice Glutelin Subunits.- Evaluating Amyloid Beta (Ab) 1-40 Degradation by Capillary Electrophoresis.- Capillary Electrophoresis for Protein Profiling of the Dimorphic, Pathogenic Fungus, Penicillium marneffei.- Capillary Electrophoretic Enzyme Assays.- A Methodology for Detection and Quantification of Esterase Activity.- Enzyme Inhibitor Screening by CE with an on-Column Immobilized Enzyme Microreactor Created by an Ionic Binding Technique.- Fluorescent Lipids as Probes for Sphingosine Kinase Activity by Capillary Electrophoresis.- Assessment of DNA damage by Micellar Electrokinetic Chromathography.Approaches to Enhancing the Sensitivyty of Carbohydrate Separations in Capillary Electrophoresis.- Determination of Monosaccharides Derivatized with 2-Aminobenzoic Acid by Capillary Electrophoresis.- Determination of Mono- Di- and Oligosaccharides by Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection.- Separation of Chitooligosaccharides in Acidic Solution by Capillary Electrophoresis.- Capillary Electrophoresis for the Analysis of Glycosaminoglycan-derived Disaccharides.- High Throughput Capillary Electrophoresis Mass Spectrometry: From Analysis of Amino Acids to Analysis of Protein Complexes.- Separation of Amino Acids by Capillary Electrophoresis with Light-emitting Diode-induced Fluorescence in the Presence of Electroosmotic Flow.- Quantification of Arginine and Dimethylated Arginines in Human Plasma by Field-amplified Sample Injection Capillary Electrophoresis UV Detection.- Capillary Electrophoresis-Mass Spectrometry for Peptide Analysis: Target-Based Approaches and Proteomics/Peptidomics Strategies.- High Resolution Proteome/peptidome Analysis of Body Fluids by Capillary Electrophoresis Coupled with MS.- Contribution of CE to the Analysis of Protein or Peptide Biomarkers.- Highly Charged Polyelectrolyte Coatings to Prevent Adsorption During Protein and Peptide Analysis in Capillary Electrophoresis.- Capillary Electrophoresis with Laser Induced Fluorescence Detection of Proteins from Two Types of Complex Sample Matrices: Food and Biological Fluids.- Automated Capillary Electrophoresis in the Screening for Haemoglobinopathies.- Protein Fingerprinting of Staphylococcus aureus by Capillary Electrophoresis with On-capillary Derivatization and Laser-induced Fluorescence Detection.- Capillary Electrophoresis of Seed Storage Proteins: The Separation and Identification of Microheterogeneous Rice Glutelin Subunits.- Evaluating Amyloid Beta (Ab) 1-40 Degradation by Capillary Electrophoresis.- Capillary Electrophoresis for Protein Profiling of the Dimorphic, Pathogenic Fungus, Penicillium marneffei.- Capillary Electrophoretic Enzyme Assays.- A Methodology for Detection and Quantification of Esterase Activity.- Enzyme Inhibitor Screening by CE with an on-Column Immobilized Enzyme Microreactor Created by an Ionic Binding Technique.- Fluorescent Lipids as Probes for Sphingosine Kinase Activity by Capillary Electrophoresis.- Assessment of DNAdamage by Micellar Electrokinetic Chromathography.