Techniques and Protocols in Molecular Biology

Auteur
Dr. Buddhi Prakash Jain is an Assistant Professor at the Department of Zoology, Mahatma Gandhi Central University, Motihari, Bihar, India. He completed his doctoral studies at the School of Life Sciences, Jawaharlal Nehru University (JNU) New Delhi, India. His research interests include gene expression regulation and endoplasmic reticulum stress in cancer. He is the recipient of several awards including a gold medal in Master Programme (M.Sc.), EMBO travel award, DST (Department of Science and Technology) Govt of India, Young Scientist travel award, and has published several papers in reputed international journals and a number of books and book chapters.

Texte du rabat

Techniques and Protocols in Molecular Biology delivers detailed practical guidance for a variety of molecular techniques and procedures. Each chapter focuses on a different technique, including isolation techniques for DNA and RNA, gene cloning, PCR, DNA sequencing, ELISA and cell culture. These protocols all includes a detailed description of the technique along with the main principles, required equipment, and comprehensive steps to assist researchers with replicating the protocol and performing the techniques themselves.

This foundational reference is essential for students and researchers looking to enhance their understanding of key molecular techniques and reproduce them successfully in their own laboratory work.

Contenu

1. Isolation of Genomic DNA: From animal cells, tissues, plant cell, and bacteria 2. Isolation of Plasmid 3. Isolation of RNA 4. PCR (Polymerase Chain reaction) 5. Real-time (Quantitative) PCR 6. Electrophoresis 7. Capillary electrophoresis 8. Purification of Nucleic acid 9. Southern blotting 10. Northern blotting 11. ELISA (Enzyme Linked Immuno Sorbent Assay) 12. EMSA (Electrophoretic Mobility Shift Assay) 13. Gene Cloning 14. End labelling of DNA 15. DNA sequencing 16. RNA sequencing 17. Microarray 18. Generation of Genomic and cDNA library. 19. Western Blotting 20. Cell Culture 21. Fluorescent activated cell sorting (FACS) 22. DNA microarray 23. DNA foot printing 24. Chromatin Immunoprecipitation 25. Basics of Bioinformatics 26. Isolation of Proteins 27. Protein purification 28. Fluorescence In situ Hybridization