Brain Organoid Research

This volume explores multiple methods and approaches used to generate human brain and neuroretinal organoids to address fundamental questions in human brain research. The chapters in this book cover topics such as self-organizing brain organoids with ventricles amenable to injection and electroporation; visualization of 3D organoids though the latest advancements in microscopy; generation of 3D retinal tissue with physiologically competent, light-sensitive photoreceptors; modeling brain tumors using genetically edited brain organoids; and brain organoids as a model to study Zika virus and SARS-CoV-2 infections. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Comprehensive and cutting-edge, Brain Organoid Research is a valuable resource for researchers at various levels of learning, ranging from undergraduate students, early researchers, and advanced laboratories. This book aims to be instrumental in moving this developing field forward.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Contenu
Self-Organizing Brain Organoids with Ventricles Amenable to Injection and Electroporation.- Profiling Cell Type-Specific Gene Regulatory Regions in Human Cortical Organoids.- Visualization of 3D Organoids Through the Latest Advancements in Microscopy.- Generation and Isolation of Retinal Ganglion Cells and Photoreceptors from Human iPSC-Derived Retinal Organoids by Magnetic-Activated Cell Sorting.- Micromanipulator-Assisted Subretinal Transplantation of Human Photoreceptor Reporter Cell Suspensions into Mice.- Generation of Three-Dimensional Retinal Tissue with Physiologically Competent, Light Sensitive Photoreceptors from Human Induced Pluripotent Stem Cells.- Quantitative Assessment of Fluorescent Reporter Expression in 3D Retinal Organoids.- A Three-Dimensional Organoid Culture System to Model Invasive Patterns of Patient-Derived Glioma Stem Cells.- Modeling Brain Tumors using Genetically Edited Brain Organoids.- Brain Organoids as aModel to Study Zika Virus and SARS-CoV-2 Infections.- Infection of 3D Brain Organoids with Human Pathogenic Viruses Under Biosafety Level 3 Conditions with Subsequent Inactivation to Study Viral Replication, Pathomechanisms, and Other Viral Infection-Mediated Effects.