Biocatalysis Based on Heme Peroxidases

This book presents recent advances on evolution, structure-function relation and catalytic mechanism as well as applied aspects such as bioreactor and protein engineering. It will help in the design of enhanced biocatalysts and biocatalytic processes.

The last systematic description of heme peroxidases was published in 1999 by Brian Dunford, from the University of Alberta in Canada. The book Heme per- idases covers discussion on three-dimensional structure, reaction mechanism, kinetics, and spectral properties of representative enzymes from bacterial, plant, fungal, and animal origin. Since 1999, vast information on basic but also applied aspects of heme peroxidases has been generated. We believe fusion of these two aspects will bene?t research of those dedicated to development of biocatalytic process. The aim of this book is to present recent advances on basic aspects such as evolution, structurefunction relation, and catalytic mechanism, as well as applied aspects, such as bioreactor and protein engineering, in order to provide the tools for rational design of enhanced biocatalysts and biocatalytic processes. The book does not include an exhaustive listing of references but rather a selected collection to enrich discussion and to allow envisioning future directions for research. This book is organized in three parts. In Part I, current knowledge of structure and mechanism of peroxidases is covered. From the molecular phylogeny, going through the in?uence of structural factors over oxidative ability to the molecular mechanism of catalysis, the authors intend to provide an understanding of per- idases at the molecular level. The understanding of the fundamental behavior of peroxidases will allow further adequation, design, and/or optimization of pero- dase-based catalysis to a particular process. In Part II, research on potential applications of peroxidases in several ?elds is presented and discussed.

First monograph on the subject for 10 years Especially covers the application in the biotechnology industry including the limitations summarizes new data. Includes supplementary material: sn.pub/extras

Texte du rabat
This monograph describes many applications of peroxidase-based biocatalysis in the biotechnology industry. The need for such a book emerges from the considerable amount of new data regarding the phylogeny, reaction mechanisms, thermodynamic characterization and structural features of fungal and plant heme peroxidases that has been generated in the past 10 years, since the last specialized book on peroxidases was published. The aim of this book is to present recent advances on such basic aspects as evolution, structure-function relation and catalytic mechanism as well as applied aspects, such as bioreactor and protein engineering, to provide the tools for rational design of enhanced biocatalysts and biocatalytic processes.

Contenu
Molecular and Structural Aspects of Peroxidases.- Molecular Phylogeny of Heme Peroxidases.- Structural and Functional Features of Peroxidases with a Potential as Industrial Biocatalysts.- Redox Potential of Peroxidases.- Catalytic Mechanisms of Heme Peroxidases.- Prospective Usage of Peroxidases in Industry.- Potential Applications of Peroxidases in the Fine Chemical Industries.- Grafting of Functional Molecules: Insights into Peroxidase-Derived Materials.- Applications and Prospective of Peroxidase Biocatalysis in the Environmental Field.- Challenges in the Application of Peroxidases.- Enzyme Technology of Peroxidases: Immobilization, Chemical and Genetic Modification.- Reactor Engineering.- Deactivation of Hemeperoxidases by Hydrogen Peroxide: Focus on Compound III.- Heterologous Expression of Peroxidases.- A Compendium of BioPhysicalChemical Properties of Peroxidases.