Bacterial and Archaeal Motility

This detailed volume presents cutting-edge research protocols to study the structure and dynamics of bacterial and archaeal motility systems using bacterial genetics, molecular biology, biochemistry, biophysics, structural biology, cell biology, microscopy imaging, and molecular dynamics simulation. Beginning with a section on bacterial flagellar protein export and assembly, the book continues with chapters covering flagella-driven motility of bacteria, archaella-driven motility of archaea, type IV-driven twitching motility of bacteria, as well as adhesion-based gliding motility of bacteria and other unique motility systems. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough, Bacterialand Archaeal Motility is the ideal reference for researchers working in this vital area of microbiology.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

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Purification of the Transmembrane Polypeptide Channel Complex of the Salmonella Flagellar Type III Secretion System.- In Vitro Flagellar Type III Protein Transport Assay Using Inverted Membrane Vesicles.- Molecular Simulation to Investigate OpenClose Motion of a Flagellar Export Apparatus Protein FlhA C .- Live-Cell Imaging of the Assembly and Ejection Processes of the Bacterial Flagella by Fluorescence Microscopy.- Purification and CryoEM Image Analysis of the Bacterial Flagellar Filament.- Site-Specific Isotope Labeling of FliG for Studying Structural Dynamics Using Nuclear Magnetic Resonance Spectroscopy.- Site-Directed Crosslinking between Bacterial Flagellar Motor Proteins In Vivo.- Measurements of the Ion Channel Activity of the Transmembrane Stator Complex in the Bacterial Flagellar Motor.- Purification of the Na + -Driven PomAB Stator Complex and Its Analysis Using ATR-FTIR Spectroscopy.- Purification of Na + -Driven MotPS Stator Complexes and Single-Molecule Imaging by High-Speed Atomic Force Microscopy.- High-Resolution Rotation Assay of the Bacterial Flagellar Motor Near Zero Loads Using a Mutant Having a Rod-Like Straight Hook.- Live-Cell Fluorescence Imaging of Magnetosome Organelle for Magnetotaxis Motility.- Swarming Motility Assays in Salmonella.- Analysis of Adhesion and Surface Motility of a Spirochete Bacterium.- Force Measurement of Bacterial Swimming Using Optical Tweezers.- Archaella Isolation.- Direct Observation of Archaellar Motor Rotation by Single-Molecular Imaging Techniques.- In Situ Structure Determination of Bacterial Surface Nanomachines Using Cryo-Electron Tomography.- Twitching Motility Assays of Lysobacter enzymogenes OH11 Under a Light Microscope.- Live Cell Imaging of the Twitching Motility of Cyanobacteria by High-Resolution Microscopy.- Isolation and Visualization of Gliding Motility Machinery in Bacteroidota .- Live Cell Imaging of Gliding Motility of Flavobacterium johnsoniae Under High-Resolution Microscopy.- Social Motility Assays of Flavobacterium johnsoniae .- Visualization of Peptidoglycan Structures of Escherichia coli by Quick-Freeze Deep-Etch Electron Microscopy.- Purification and Structural Analysis of the Gliding Motility Machinery in Mycoplasma mobile .- Motility Assays of Mycoplasma mobile Under Light Microscopy.- Detection of Steps and Rotation in the Gliding Motility of Mycoplasma mobile .- Direct Measurement of Kinetic Force Generated by Mycoplasma.- Genetic Manipulation of Mycoplasma pneumonia .- Purification and ATPase Activity Measurement of Spiroplasma MreB.- Swimming Motility Assays of Spiroplasma.- Motility Assays of Chloroflexus.