Atherosclerosis

This volume provides detailed, up-to-date methods used in research on Atherosclerosis. Chapters guide readers through an overview of the pathogenesis of atherosclerosis and model systems together with in vitro , ex vivo , in vivo and emerging methods in atherosclerosis research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Atherosclerosis: Methods and Protocols serves as an invaluable resource for those engaging in research on atherosclerosis and cardiovascular disease, as well as for researchers who are new to the field and students at both the undergraduate and postgraduate level.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Texte du rabat
This volume provides detailed, up-to-date methods used in research on Atherosclerosis. Chapters guide readers through an overview of the pathogenesis of atherosclerosis and model systems together with in vitro, ex vivo, in vivo and emerging methods in atherosclerosis research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Atherosclerosis: Methods and Protocols serves as an invaluable resource for those engaging in research on atherosclerosis and cardiovascular disease, as well as for researchers who are new to the field and students at both the undergraduate and postgraduate level.

Contenu
Atherosclerosis: Pathogenesis and key cellular processes, current and emerging therapies, key challenges and future research directions.- Key roles of inflammation in atherosclerosis: Mediators involved in orchestrating the inflammatory response and its resolution in the disease along with therapeutic avenues targeting inflammation.- Survey of in vitro model systems for investigation of key cellular processes associated with atherosclerosis.- Survey of approaches for investigation of atherosclerosis in vivo.- Exploring the associations between clonal hematopoiesis of indeterminate potential, myeloid malignancy and atherosclerosis.- Peptides as therapeutic agents for atherosclerosis.- Isolation and culturing of primary mouse and human macrophages.- Effective transfection and gene silencing of primary murine macrophages with small interfering RNA.- Monitoring celular proliferation, migration and apoptosis associated with atherosclerosis plaques invitro.- Monocyte adhesion assays for detecting endothelial cell activation in vascular inflammation and aterosclerosis.- Chick embryo ex vivo aortic sprouting assays for cardiovascular research.- Purification and analysis of circulating lipid particles.- Preparation of LDL, oxidation, methods of detection and applications in atherosclerosis research.- Monitoring modified lipoprotein uptake and macropinocytosis associated with macrophage foam cell formation.- A fluorescent based in vitro method to assess cholesterol efflux.- Monitoring the anisotropy and fluidity of the HDL monolayer as surrogates of HDL functionality.- Assessment of ex-vivo antioxidative potential of murine HDL in atherosclerosis.- Dual-fluorescence in vitro efferocytosis assay.- Investigation of mitochondrial bioenergetic profile and dysfunction in atherosclerosis.- Probing inflammasome activation in atherosclerosis.- Investigation of platelet aggregation in atherosclerosis.- Extracellular vesicles in atherosclerosis research.- The use of wire myography to investigate vascular tone and function.- Pig and mouse models of hyperlipidemia and atherosclerosis.- Use of rabbit models to study atherosclerosis.- Genetically engineered hamster models of dyslipidemia and atherosclerosis.- Atherosclerosis induced by adeno-associated virus encoding gain-of-function PCSK9.- Monitoring leukocyte migration during atherosclerosis in vivo.- Evaluation of plaque burden and lipid content in atherosclerotic plaques.- Monitoring cellularity and expression of key markers in atherosclerotic plaques.- Monitoring cellular proliferation and apoptosis in atherosclerosis plaques and intimal thickenings.- Investigation of atherosclerotic plaque vulnerability.- Use of mouse carotid artery ligation model of intimal thickening to probe vascular smooth muscle cell remodelling and function in atherosclerosis.- Assessing efferocytosis in atherosclerotic lesions in situ.- Monitoring atheroprotective macrophage cholesterol efflux in vivo.- Flow cytometry analysis of hematopoietic stem/progenitor cells and mature blood cell subsets in atherosclerosis.- Whole mount preparation of mouse aorta for confocal microscopy studies of the intima.- Isolation of mouse aortic RNA for transcriptomics.- Redox proteomics analysis of atherosclerotic aortas: Application of the OxICAT method.- Intravital microscopy in atherosclerosis research.- Localization of long non-coding RNA in formalin-fixed-paraffin embedded vascular tissue using in-situ hybridization.- In vivo gene editing in lipid and atherosclerosis research.- Laser capture microdissection-based mRNA expression microarrays and single-cell RNA sequencing in atherosclerosis research.- Combined single cell RNA and single cell / T cell receptor sequencing of the arterial wall in atherosclerosis.- Tissue clearing approaches in atherosclerosis.- Single cell antibody sequencing in atherosclerosis research.- Flow cytometry and mass cytometry for measuring the immune cell infiltrate in atherosclerotic arteries.- Contrast-enhanced ultrasound molecular imaging in atherosclerosis.- Nanoprobes for computed tomography and magnetic resonance imaging in atherosclerosis research.- Positron emission tomography in atherosclerosis research.- Three-dimensional visualization of atherosclerotic vessels by tissue clearing and light sheet fluorescence microscopy.- Intravascular fluorescence molecular imaging of atherosclerosis.