Antibody Expression and Production

Engineered antibodies currently represent over 30% of biopharmaceuticals in clinical trials and their total worldwide sales continue to increase significantly. The importance of antibody applications is reflected in their increasing clinical and industrial applications as well as in the progression of established and emerging production strategies. This volume provides detailed coverage of the generation, optimization, characterization, production and applications of antibody. It provides the necessary theoretical background and description of methods for the expression of antibody in microbial and animal cell cultures and in transgenic animals and plants. There is a strong focus on those issues related to the production of intrabodies, bispecific antibody and antibody fragments and also to novel applications in cancer immunotherapy.

Detailed background for antibody generation and production Chapters on production of antibody in bacterial, fungal, mammalian, insects and transgenic animal and plants Scientific and technical approaches to accelerating the development of robust upstream and downstream processes Identify the limitations in developing processes for antibody production

Contenu

1 Expression of antibody in mammalian cells, Thomas Jostock.- 2 Bioreactor systems for producing antibody from mammalian cells, Darrin Kuystermans and Mohamed Al-Rubeai.- 3 Production of antibody in insect cells, Hideki Yamaji.- 4 Production of monoclonal antibodies in glycoengineered Pichia pastoris, Dongxing Zhu, Thomas Linden and Thomas Potgieter.- 5 Production of antibodies in Hansenula polymorpha, Georg Melmer, Stephan Hellwig, Grit Hehmann and Ulrike Dahlems.- 6 Expression of antibody in transgenic avians, Yoshinori Kawabe and Masamichi Kamihira.- 7 Production of antibodies in plants, Alejandro Sarrion-Perdigones, Paloma Juarez, Antonio Granell and Diego Orzaez.- 8 Production of antibody fab fragments in Escherichia coli, Hiroshi Tachibana and Masataka Takekoshi.- 9 Intrabody expression in mammalian cells, Silvia Biocca.- 10 Engineering of antibodies for cancer therapy, Lisa E. Goldsmith and Matthew K. Robinson.- 11 Recombinant bispecific antibodies for cancer therapy, Dafne Muller and Roland Kontermann.- 12 The role of glycosylation in therapeutic antibodies, Maureen Spearman, Ben Dionne and Mike Butler.- 13 Quality issues arising from post-translational modification of recombinant antibodies, Raymond Tyther and Nigel Jenkins.- 14 Purification of antibody, XueJun Han, Arther Hewig and Ganesh Vedantham.- Index