Analytical Ultracentrifugation VI

This volume includes 20 contributions of the 12th meeting on Analytical Ultracentrifugation from March 1-2, 2001 in Duisburg, Germany. Various fields of ultracentrifugation are covered concerning research problems in biochemistry, biophysical chemistry and macromolecular chemistry as well as interacting systems. New investigations concerning the sedimentation theory are presented. The phase transition of gels is dealt with, as is the sedimentation-diffusion equilibrium of gels. One section contains the hydrodynamics of biopolymers.

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Contenu
Technical and Methodological Investigations.- Evaluation of equilibrium and nonequilibrium density gradients in an analytical ultracentrifuge by calibration with marker particles.- Solubility equilibrium gradients in the analytical ultracentrifuge: an approach towards the isolation of critical crystal nuclei in solution.- Polymers, Colloids and Supramolecular Systems.- Hybrid polymer heterogeneity and boundary profile in a low-speed sedimentation experiment.- Studies on the partial specific volume of a poly(ethylene glycol) derivative in different solvent systems.- Characterization of serum albumin nanoparticles by sedimentation velocity analysis and electron microscopy.- Properties of a membrane-forming polymer, poly(1-trimethylgermyl-1-propyne), in dilute solutions.- Biological Systems.- Combination of preparative and analytical ultracentrifugation methods for the investigation of low-density lipoprotein aliquots to prepare radioactive-labelled lipoproteins.- Physicochemical and chemical characterisation of chitosan in dilute aqueous solution.- Protein hydration varies with protein crowding and with applied pressure: a sedimentation velocity study.- Determination of thermodynamic properties of sodium alginate from bacteria and seaweeds in aqueous solutions.- Interacting Systems.- Self-association of phosphorylase kinase from rabbit skeletal muscle in the presence of natural osmolyte, trimethylamine N-oxide.- The state of association of the cytochrome bc1 complex from Paracoccus denitrificans in solutions of dodecyl maltoside.- Study of polyelectrolyte complex formation applying the synthetic boundary technique of analytical ultracentrifugation.- Thermodynamics.- The sedimentation behaviour of gels the generalised Lamm's differential equation.- Evidence for phasetransitions of aqueous gelatin gels in a centrifugal field.- Exact theory of sedimentation equilibrium made useful.- Hydrodynamics.- Correlations between crystallographic, small-angle scattering and hydrodynamic data of biopolymers.- Modeling of protein hydration with respect to X-ray scattering and hydrodynamics.- Prediction of hydrodynamic parameters of Lumbricus terrestris hemoglobin from small-angle X-ray and electron microscopic structures.- Evaluation of draining and volume effects in the interpretation of hydrodynamic data for linear macromolecules.