Our study was designed to detect and evaluation Ampicillin class C B- lactamases genes from Escherichia coli isolates in patients with UTI by using of PCR technique . A total of 130 (52%) samples with significant bacteriuria were detected. The study showed higher incidence of UTI in females (73.08%) than males (26.9%).The bacteria which were grown on culture of all the 130 urine samples with significant bacteriauria were as follows: 60 ( 46.2 %) isolates of E. coli and 70 ( 53.8% ) were other isolates such as P. aerogenosa , Proteus mirabilis , K. pneumonia and Staphylococcus aureus. The results showed that only 18 (34%) isolates gave positive results with bla AmpC gene and all bla AmpC positive isolates showed diversity presence of plasmid-mediated AmpC B-lactamases. The genes t h a t encoded FOX,CIT, DHA, EBC, ACC and MOX were found in the percentage of 44.4, 38.9, 27.8, 50.0, 22.2 and 27.8, respectively.

Autorentext

MSc Abeer mohammed Ali, Department of Microbiology, college of science,University of Al-Muthana-Iraq. Brith date:8/4/1986. Assist.lecturer in University of Al-Muthana/college of science.