Usage of NMR Spectroscopy in Profiling Metabonomics

Sadik Mejid

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Presentation slides from the year 2016 in the subject Chemistry - Organic Chemistry, grade: 1,0, University of Cologne (Organisch...

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Presentation slides from the year 2016 in the subject Chemistry - Organic Chemistry, grade: 1,0, University of Cologne (Organische Chemie), language: English, abstract: Presentation with the title "Usage of NMR Spectroscopy in Profiling/ metabonomics" and the following contents: - NMR Spectroscopy; - Spin-spin coupling; - Why 1H NMR is adequate for the analysis of fruit juices; - Usage of Amix viewer for Data Analysis; - Objectives; - Preparation of phosphate buffer (pH 6 - 7 at 25°C); - Sample Preparation; - Main experiment (noesypr) (nuclear Overhauser effect spectroscopy Presaturation); - Why the Identification of Metabolites; - Organic acids occuring in Fruit juices; - Sugars and amino acids occuring in fruit juices; - Fruit juices; - Identification of metabolites in the extrakted apple juice; - Identification of metabolites in the commercial apple juices; - Investigation of the vitamine C content; - Mixing of unripe fruits in apple juices; - Identification of metabolites in the orange juice; - Identification of metabolites in the multivitamine juice; - Sugar content in commertial fruit juices; - Changes in the fruit juice samples after three weeks; - Investigation of some Coca Cola brands; - Sugar free Cola (discount brand); - Summary