Manual on general laboratory handling techniques in plant pathology

Ritesh Yadav

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Document from the year 2019 in the subject Forestry / Forestry Economics, grade: 9.0, , course: Plant Pathology, language: Englis...

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Document from the year 2019 in the subject Forestry / Forestry Economics, grade: 9.0, , course: Plant Pathology, language: English, abstract: This book is a manual on general laboratory handling techniques in plant pathology. It will present different conditions that are essential for those who are interested in working in the field of plant pathology in a laboratory. The analysis in this book focuses on various circumstances like general requirements, laboratory equipments, sterilization techniques, the isolation of bacteria etc. When designing a laboratory there are many aspects to consider. It is important that work should be carried out in a logical order and, that particular parts of the diagnostic protocol are separated from one another. General plant protection laboratory may have the following different rooms and chamber as appropriate. The preparation room is used for preparing media, including sterilizing items in the autoclave, sterilizing petri dishes in an oven, washing glassware and storing glassware, chemicals and other basic items. This room should have an exhaust fan to remove hot air produced by the autoclave and the oven. The clean room is used for isolating fungi and bacteria from cleaned subsamples of diseased plant tissue into pure cultures. It is also used for growing cultures under clean conditions. The microscopes are located in this room for examining cultures and fungal structures. This room should be air-conditioned, if possible, to protect equipment and cultures. It should also be kept free from dust and insects. If, do not have an airtight clean room or humidity will be too high and fungus (mould) will develop on walls and equipment. A dehumidifier is useful in this room. No soil is allowed in the clean room as soil is a source of fungus-eating mites that can contaminate cultures.